LGALS1 Mouse

Galectin-1 Mouse Recombinant
Cat. No.
BT10089
Source
Escherichia Coli.
Synonyms
Galectin-1, Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1, Lgals1, Gbp, L14, Galbp, L-14.5, Lect14, AA410090.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LGALS1 mouse Recombinant produced E. coli is a single polypeptide chain containing 159 amino acids (1-135) and having a molecular mass of 17kDa.
LGALS1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Galectins are a family of proteins that bind to beta-galactosides. They are involved in how cells interact with each other and with the extracellular matrix. Galectin-1, a member of this family, acts as a negative growth factor, regulating cell proliferation, apoptosis, and differentiation. It interacts with cell surface receptors like CD45, CD3, and CD4, influencing immune responses by affecting T-cell function. Galectin-1's role in tumor development is complex, with its presence often correlating with tumor progression and metastasis. However, it also shows promise in treating inflammation, neurodegenerative diseases, and muscular dystrophies. This makes galectin-1 a potential target for developing new therapies, with its inhibition being explored for cancer treatment and its enhancement for other conditions.
Description
Recombinant LGALS1 from mouse, produced in E. coli, is a single polypeptide chain of 159 amino acids (amino acids 1-135) with a molecular weight of 17 kDa. It includes a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The LGALS1 solution has a concentration of 1 mg/ml and is formulated in 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeatedly freezing and thawing the solution.
Purity
The purity is determined to be greater than 95.0% using SDS-PAGE analysis.
Synonyms
Galectin-1, Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1, Lgals1, Gbp, L14, Galbp, L-14.5, Lect14, AA410090.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMACGLV ASNLNLKPGE CLKVRGEVAS DAKSFVLNLG KDSNNLCLHF NPRFNAHGDA NTIVCNTKED GTWGTEHREP AFPFQPGSIT EVCITFDQAD LTIKLPDGHE FKFPNRLNME AINYMAADGD FKIKCVAFE.

Product Science Overview

Introduction

Galectin-1 is a member of the galectin family, which consists of carbohydrate-binding proteins with a high affinity for β-galactoside-containing glycoconjugates. This protein is encoded by the LGALS1 gene and is characterized by its ability to bind to specific sugar moieties on the surfaces of cells and within the extracellular matrix .

Structure and Properties

Galectin-1 is a 135 amino acid, 14 kDa protein that can exist as a monomer or homodimer. It lacks a classical signal peptide, which means it can be localized to the cytosolic compartments or secreted via non-classical pathways . The recombinant form of mouse Galectin-1 is typically produced in Escherichia coli and purified to a high degree of purity, often greater than 95% as determined by SDS-PAGE .

Biological Functions

Galectin-1 plays a crucial role in various biological processes, including cell-cell adhesion, cell-matrix interactions, and immune response modulation. It has been shown to have immunosuppressive and anti-inflammatory properties, making it a key player in the resolution of acute and chronic inflammation . Galectin-1 can inhibit the synthesis of proinflammatory cytokines, reduce neutrophil trafficking, and suppress mast cell degranulation .

Therapeutic Potential

Due to its broad anti-inflammatory and immunomodulatory activities, Galectin-1 has been studied for its potential therapeutic applications in various diseases. These include autoimmune diseases, allergic inflammation, cancer, and infections . Therapeutic strategies targeting Galectin-1 interactions with glycans could help overcome cancer immunosuppression and enhance antimicrobial immunity .

Applications in Research

Recombinant mouse Galectin-1 is widely used in research to study its biological functions and therapeutic potential. It is often used in cell culture experiments, ELISA assays, and other biochemical analyses. The protein is typically lyophilized and reconstituted in sterile PBS for use in various experimental setups .

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LGALS1 Human
Galectin-1 Human Recombinant
Cat. No.
BT9991
Source
Escherichia Coli.
THE BIOTEK
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