LDHA Antibody
Lactate Dehydrogenase A, Mouse Anti Human
Cat. No.
BT20601
Source
Synonyms
LDH-A, GSD11, LDH1, LDHM, PIG19, EC 1.1.1.27, lactate dehydrogenase M, LDH-M, LDH-1, L-lactate dehydrogenase A chain, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, Cell proliferation-inducing gene 19 protein, LDHA.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Lactate dehydrogenase A (LDHA) is an enzyme that catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. It is found in a variety of organisms, including animals, plants, and bacteria. LDHA is a tetrameric enzyme, meaning that it is composed of four subunits. Each subunit has a molecular weight of approximately 35 kDa. LDHA is a key enzyme in anaerobic glycolysis, which is the process by which cells produce energy from glucose in the absence of oxygen. In addition to its role in anaerobic glycolysis, LDHA is also involved in a number of other cellular processes, including gluconeogenesis, the pentose phosphate pathway, and the malate-aspartate shuttle. LDHA has been implicated in a number of diseases, including cancer, obesity, and diabetes. Inhibitors of LDHA are being investigated as potential treatments for these diseases.
Formulation
This product is supplied as a 1mg/ml solution in PBS, pH 7.4, containing 0.1% sodium azide.
Storage Procedures
This antibody should be stored at 4°C for short-term storage (up to 1 month) or -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
This antibody is stable for 12 months from the date of receipt when stored at -20°C. It is stable for 1 month when stored at 4°C.
Applications
This antibody can be used in ELISA, Western blot, and immunofluorescence applications. The recommended dilution range for Western blotting and immunofluorescence is 1:500-8,000. The recommended starting dilution for all applications is 1:1,000.
Synonyms
LDH-A, GSD11, LDH1, LDHM, PIG19, EC 1.1.1.27, lactate dehydrogenase M, LDH-M, LDH-1, L-lactate dehydrogenase A chain, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, Cell proliferation-inducing gene 19 protein, LDHA.
Purification Method
LDHA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1A4AT.
Immunogen
Anti-human LDHA mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human LDHA amino acids 1-332 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Mouse Anti-Human Antibodies
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins . These antibodies are highly specific for human proteins and are widely used in various research and diagnostic applications, including ELISA, Western Blot, Flow Cytometry, and Immunohistochemistry .
Production and Applications
The production of mouse anti-human antibodies involves immunizing mice with human antigens, followed by the collection and purification of the resulting antibodies . These antibodies can be conjugated with various labels, such as enzymes or fluorophores, to facilitate detection and quantification of target proteins . They are essential tools in biomedical research, enabling the study of human proteins in various experimental settings .
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