LCP2 Human

Lymphocyte Cytosolic Protein 2 Human Recombinant

Cat. No.

BT21361

Source

Sf9, Baculovirus cells.

Synonyms

Lymphocyte Cytosolic Protein 2, SH2 Domain-Containing Leukocyte Protein Of 76 KDa, 76 KDa Tyrosine Phosphoprotein, SLP-76 Tyrosine Phosphoprotein, SLP76, Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein Of 76kDa), Lymphocyte Cytosolic Protein 2 (SH2 Domain-Containing Leukocyte Protein Of 76kD), SH2 Domain-Containing Leukocyte Protein Of 76Kd, SLP-76, LCP2.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

LCP2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (1-533 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 542 amino acids and having a molecular mass of 61.2kDa.
LCP2 is purified by proprietary chromatographic techniques.

Product Specs

Introduction

Lymphocyte cytosolic protein 2 (LCP2) is an intracellular docking protein that plays a crucial role in T-cell antigen receptor signaling. It contains a single SH2 recognition domain, which is essential for its function. LCP2 is phosphorylated by ZAP70, leading to the activation of NF-AT and IL2 gene expression, both of which are critical for T-cell activation. Deficiency in LCP2 is associated with B Cell Linker Protein Deficiency, highlighting its importance in immune regulation.

Description

Recombinant LCP2 protein, expressed in Sf9 Baculovirus cells, is a single polypeptide chain with a molecular weight of 61.2 kDa. It encompasses amino acids 1-533 of the human LCP2 protein and includes a 6-amino acid Histidine tag at the C-terminus for purification purposes. The protein is glycosylated and purified using proprietary chromatographic techniques, resulting in a highly pure product.

Physical Appearance

The LCP2 protein solution is sterile-filtered and colorless.

Formulation

The LCP2 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution is formulated with 10% glycerol and Phosphate buffered saline at pH 7.4 to ensure protein stability and optimal storage conditions.

Stability

For short-term storage (2-4 weeks), the LCP2 protein solution should be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To further enhance protein stability during long-term storage, adding a carrier protein like HSA or BSA at a concentration of 0.1% is advised. To prevent protein degradation, avoid repeated freeze-thaw cycles.

Purity

The purity of the LCP2 protein is greater than 90% as determined by SDS-PAGE analysis, indicating a highly pure product suitable for various research applications.

Synonyms

Source

Sf9, Baculovirus cells.

Amino Acid Sequence

ADPMALRNVP FRSEVLGWDP DSLADYFKKL NYKDCEKAVK KYHIDGARFL NLTENDIQKF PKLRVPILSK LSQEINKNEE RRSIFTRKPQ VPRFPEETES HEEDNGGWSS FEEDDYESPN DDQDGEDDGD YESPNEEEEA PVEDDADYEP PPSNDEEALQ NSILPAKPFP NSNSMYIDRP PSGKTPQQPP VPPQRPMAAL PPPPAGRNHS PLPPPQTNHE EPSRSRNHKT AKLPAPSIDR STKPPLDRSL APFDREPFTL GKKPPFSDKP SIPAGRSLGE HLPKIQKPPL PPTTERHERS SPLPGKKPPV PKHGWGPDRR ENDEDDVHQR PLPQPALLPM SSNTFPSRST KPSPMNPLPS SHMPGAFSES NSSFPQSASL PPYFSQGPSN RPPIRAEGRN FPLPLPNKPR PPSPAEEENS LNEEWYVSYI TRPEAEAALR KINQDGTFLV RDSSKKTTTN PYVLMVLYKD KVYNIQIRYQ KESQVYLLGT GLRGKEDFLS VSDIIDYFRK MPLLLIDGKN RGSRYQCTLT HAAGYPHHHH HH

Product Science Overview

Structure and Function

LCP2 is composed of three modular domains:

N-terminal domain: Contains an acidic region with sections for SH2-domain binding and tyrosine residues that bind proteins like Vav and Nck when phosphorylated.
Central domain: Rich in prolines and binds SRC-Homology 3 (SH3) domains of other adaptor molecules such as Grb2 and Gads.
C-terminal domain: Involved in forming larger signaling complexes.

As an adaptor protein, LCP2 does not have catalytic functions but instead binds other signaling proteins to facilitate the formation of larger signaling complexes. This is essential for the proper functioning of T-cell receptors and the immune response.

Expression and Localization

LCP2 is expressed in various immune cells, including T cells, natural killer cells, and myeloid cells. It is predominantly found in the cytoplasm and cytosol, but it can also be localized to the plasma membrane raft and cell-cell junctions.

Biological Processes

LCP2 is involved in several key biological processes, including:

T-cell receptor signaling pathway: Essential for T-cell activation and immune response.
Intracellular signal transduction: Facilitates communication within cells to trigger appropriate responses.
Positive regulation of protein kinase activity: Enhances the activity of protein kinases, which are crucial for various cellular functions.

Clinical Relevance

Mutations or dysregulation of LCP2 can lead to immune-related disorders. For example, it is associated with conditions like Immunodeficiency 81 and Agammaglobulinemia 4. Understanding the role of LCP2 in these diseases can help in developing targeted therapies.

Research and Applications

Recombinant LCP2 is used in research to study its function and interactions with other proteins. It is also valuable in exploring potential therapeutic interventions for immune-related disorders.

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LCP2 Human

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