LCMT1 Human

LCMT1 is a mammalian methyltransferase that interacts with its cofactor, S-adenosylmethionine (SAM), to transfer a methyl group to the target leucine residue on PP2A . The structural integrity of LCMT1 and its complex with SAM is crucial for its interaction with PP2A, as revealed by X-ray crystallography studies . This interaction ensures the proper regulation of PP2A activity, which is vital for maintaining cellular homeostasis.

Recombinant human LCMT1 is typically expressed in baculovirus-insect cell systems, which allows for the production of high-purity, biologically active protein . The recombinant protein is often tagged with GST (Glutathione S-transferase) to facilitate purification and enhance stability . The recombinant LCMT1 retains its full-length structure and is capable of performing its methyltransferase activity, making it a valuable tool for research and therapeutic applications.

Recombinant LCMT1 is widely used in biochemical and cellular studies to understand the regulation of PP2A and its role in various diseases, including cancer and neurodegenerative disorders . By studying the methylation process mediated by LCMT1, researchers can gain insights into the mechanisms of PP2A regulation and identify potential therapeutic targets for modulating its activity.

For optimal performance, recombinant LCMT1 should be stored at -70°C and handled with care to avoid repeated freeze-thaw cycles . The enzyme is typically supplied in a buffer containing Tris-HCl, NaCl, glutathione, EDTA, DTT, PMSF, and glycerol to maintain its stability and activity .