LBH Human

LBH Human Recombinant is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain. It contains 128 amino acids and has a molecular mass of approximately 14.6 kDa . The recombinant protein is fused to a 23 amino acid His-tag at the N-terminus, which aids in its purification through chromatographic techniques .

The LBH protein is essential for the proper formation of limb buds and the heart during the early stages of human development. It is involved in the regulation of gene expression and cellular differentiation, which are critical processes for the development of these organs. Mutations or dysregulation of the LBH gene can lead to developmental abnormalities and congenital defects.

The recombinant LBH protein is produced using E. coli expression systems. The protein is then purified to a high degree of purity, typically greater than 90%, using proprietary chromatographic techniques . The purified protein is formulated as a sterile filtered clear solution containing 20 mM Tris-HCl (pH 8.0), 20% glycerol, 0.15 M NaCl, and 1 mM DTT .

LBH Human Recombinant protein is used in various research applications, including:

• Developmental Biology: Studying the mechanisms of limb and heart development.
• Gene Regulation: Investigating the role of LBH in gene expression and cellular differentiation.
• Disease Models: Understanding the impact of LBH mutations on congenital defects and developmental disorders.

The LBH protein should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it is recommended to store the protein at -20°C. To ensure stability, it is advisable to add a carrier protein, such as 0.1% HSA or BSA, and avoid multiple freeze-thaw cycles .