LASP1 Human

LIM and SH3 Protein 1 Human Recombinant
Cat. No.
BT19896
Source
E.coli.
Synonyms
LIM and SH3 protein 1, MLN50, Lasp-1, Metastatic lymph node gene 50 protein.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LASP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 285 amino acids (1-261) and having a molecular mass of 32.3kDa.
LASP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
LASP1 plays a crucial role in regulating dynamic actin-based activities within the cytoskeleton. Phosphorylation of LASP1, triggered by agonists, can further contribute to the regulation of ion transport activities associated with actin in parietal cells and various other secretory epithelial cells rich in F-actin.
Description
Recombinant human LASP1, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 285 amino acids (residues 1-261). It has a molecular weight of 32.3 kDa. The LASP1 protein is expressed with a 24 amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution, sterile filtered.
Formulation
The LASP1 solution is provided at a concentration of 0.25 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity is determined to be greater than 80% by SDS-PAGE analysis.
Synonyms
LIM and SH3 protein 1, MLN50, Lasp-1, Metastatic lymph node gene 50 protein.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMNPNCA RCGKIVYPTE KVNCLDKFWH KACFHCETCK MTLNMKNYKG YEKKPYCNAH YPKQSFTMVA DTPENLRLKQ QSELQSQVRY KEEFEKNKGK GFSVVADTPE LQRIKKTQDQ ISNIKYHEEF EKSRMGPSGG EGMEPERRDS QDGSSYRRPL EQQQPHHIPT SAPVYQQPQQ QPVAQSYGGY KEPAAPVSIQ RSAPGGGGKR YRAVYDYSAA DEDEVSFQDG DTIVNVQQID DGWMYGTVER TGDTGMLPAN YVEAI

Product Science Overview

Introduction

LIM and SH3 Protein 1 (LASP1) is a member of a subfamily of LIM proteins, characterized by a LIM motif and a domain of Src homology region 3 (SH3). It is also a member of the nebulin family of actin-binding proteins . LASP1 was first identified in lymph nodes of breast cancer patients in 1995 . The gene encoding LASP1 is located on chromosome 17q11-21.3 and encodes a protein of 261 amino acids .

Structural Features

The structural analysis of LASP1 reveals a protein composed of:

  • An N-terminal LIM-domain with two zinc finger motifs
  • Two central actin-binding nebulin repeats
  • A linker region
  • A C-terminal SH3 domain

The first nebulin repeat includes a nuclear export signal (NES; aa 71–77) . The LIM domain, nebulin repeats, and SH3 domain are highly conserved among vertebrates and invertebrates .

Functions and Mechanisms

Originally identified as a structural cytoskeletal protein with scaffolding function, recent data suggest additional roles for LASP1 in cell signaling and gene expression, especially in tumor cells . These novel functions are primarily regulated by the site-specific phosphorylation of LASP1 .

Cellular Signaling

LASP1 is involved in various cellular processes such as proliferation, migration, tumorigenesis, and chemoresistance . It plays a significant role in the PI3K/AKT signaling pathway, which is crucial for cell growth and survival . LASP1 is also implicated in the regulation of chemokine receptor signaling, particularly CXCR4 .

Gene Expression

In addition to its cytoskeletal functions, LASP1 has roles in the nucleus, where it is involved in epigenetics and transcriptional regulation . It modulates oncogenic mRNA translation and interacts with various cellular proteins to orchestrate primary tumor progression and metastasis .

Clinical Significance

LASP1 is upregulated in several types of human cancer and is implicated in cancer progression . High LASP1 expression is associated with poor overall survival in glioblastoma (GBM) patients . LASP1 knockdown has been shown to suppress cell proliferation and enhance chemosensitivity to temozolomide in GBM . This makes LASP1 a promising target for therapeutic strategies in cancer treatment .

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