LANCL1 Human
Description
LANCL1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The LANCL1 gene encodes a peripheral membrane protein that is loosely associated with the cell membrane. This protein is related to the LanC family of bacterial membrane-associated proteins, which play a crucial role in the biosynthesis of lantibiotics, a class of antimicrobial peptides . The human recombinant form of LANCL1 is produced in Escherichia coli and consists of a single, non-glycosylated polypeptide chain containing 422 amino acids, with a molecular mass of approximately 47.7 kDa .
LANCL1 functions as a peptide-modifying enzyme component in eukaryotic cells. It is involved in the conjugation of glutathione (GSH) to artificial substrates such as 1-chloro-2,4-dinitrobenzene (CDNB) and p-nitrophenyl acetate. This activity suggests that LANCL1 plays a role in the glutathione antioxidant defense mechanism, helping to mitigate neuronal oxidative stress during normal postnatal development and in response to oxidative stresses .
LANCL1 is a 399-residue protein that was originally discovered in a search for binding partners to the erythrocyte protein stomatin, which is a factor in the disease hereditary overhydrated stomatocytosis . The protein binds glutathione and participates in EPS8 signaling, which is crucial for various cellular processes .
Mutations or dysregulation of the LANCL1 gene have been associated with several disorders, including intellectual developmental disorder, autosomal recessive 18, with or without epilepsy, and speech and communication disorders . The protein’s role in mitigating oxidative stress also suggests potential therapeutic applications in neurodegenerative diseases and other conditions characterized by oxidative damage.
The human recombinant form of LANCL1 is produced in Escherichia coli and is available as a sterile, filtered colorless solution. It is formulated in a buffer containing 20 mM Tris-HCl (pH 7.5), 0.15 M NaCl, 20% glycerol, and 1 mM DTT. For long-term storage, it is recommended to store the protein at -20°C with a carrier protein to avoid multiple freeze-thaw cycles .
