LAMTOR4 Human
LAMTOR4 Human Recombinant
Cat. No.
BT19723
Source
Escherichia Coli.
Synonyms
Late Endosomal/Lysosomal Adaptor, MAPK And MTOR Activator
4, C7orf59, Late Endosomal/Lysosomal Adaptor And MAPK And MTOR Activator 4, Chromosome
7 Open Reading Frame 59, Ragulator Complex Protein LAMTOR4, UPF0539 Protein
C7orf59, LAMTOR4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
LAMTOR4 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 122 amino acids (1-99 a.a) and
having a molecular mass of 13.1kDa.
LAMTOR4 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Introduction
LAMTOR4 plays a role in amino acid sensing and the activation of mTORC1, a signaling complex that promotes cell growth in response to growth factors, energy levels, and amino acids. Amino acids activate LAMTOR4 through a mechanism involving the lysosomal V-ATPase. Ragulator acts as a guanine nucleotide exchange factor, activating the small GTPases Rag. Both activated Ragulator and Rag GTPases serve as a scaffold, recruiting mTORC1 to lysosomes for sequential activation.
Description
Recombinant LAMTOR4, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 122 amino acids (amino acids 1-99). It has a molecular weight of 13.1 kDa. The LAMTOR4 protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution.
Formulation
The LAMTOR4 protein solution is provided at a concentration of 0.25 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. To ensure stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles.
Purity
The purity of the LAMTOR4 protein is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
Late Endosomal/Lysosomal Adaptor, MAPK And MTOR Activator
4, C7orf59, Late Endosomal/Lysosomal Adaptor And MAPK And MTOR Activator 4, Chromosome
7 Open Reading Frame 59, Ragulator Complex Protein LAMTOR4, UPF0539 Protein
C7orf59, LAMTOR4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTSALTQ GLERIPDQLG YLVLSEGAVL ASSGDLENDE QAASAISELV
STACGFRLHR GMNVPFKRLS VVFGEHTLLV TVSGQRVFVV KRQNRGREPI DV.
Product Science Overview
Structure and Expression
Function
LAMTOR4 is integral to the Ragulator complex, which functions as a guanine nucleotide exchange factor (GEF) for the small GTPases RagA and RagB. This complex is essential for the recruitment and activation of mTORC1 at the lysosomal surface in response to amino acids . The activation of mTORC1 is a critical step in promoting cell growth and proliferation in response to nutrient availability .
Biological Significance
The mTORC1 pathway, activated by LAMTOR4, is a central regulator of cellular metabolism, growth, and survival. Dysregulation of this pathway is implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative diseases . Therefore, understanding the function and regulation of LAMTOR4 is vital for developing therapeutic strategies targeting these conditions.
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