LAGE3 Human

L Antigen Family Member 3 Human Recombinant
Cat. No.
BT19519
Source
E.coli.
Synonyms
CVG5, DXS9879E, DXS9951E, ESO3, ITBA2, EKC/KEOPS complex subunit LAGE3, L antigen family member 3, Protein ESO-3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LAGE3 Human Recombinant produced in E. coli is a single polypeptide chain containing 166 amino acids (1-143) and having a molecular mass of 17.2kDa. LAGE3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
L antigen family member 3 (LAGE3), a member of the CTAG family, is found in most body tissues. Unlike other family members, which are usually found only in the testes and activated in various human tumors, LAGE3 is widely expressed. The high conservation of LAGE3 in mice and rats suggests its functional importance.
Description
Recombinant human LAGE3, produced in E. coli, is a single polypeptide chain with 166 amino acids (residues 1-143) and a molecular weight of 17.2 kDa. A 23 amino acid His-tag is fused to the N-terminus of LAGE3, which is then purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile solution.
Formulation
The LAGE3 solution (0.25 mg/ml) is supplied in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 50% glycerol, and 2 mM DTT.
Stability
For short-term storage (2-4 weeks), store at 4°C. For long-term storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freezing and thawing.
Purity
Purity determined by SDS-PAGE is greater than 85%.
Synonyms
CVG5, DXS9879E, DXS9951E, ESO3, ITBA2, EKC/KEOPS complex subunit LAGE3, L antigen family member 3, Protein ESO-3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMRDADAD AGGGADGGDG RGGHSCRGGV DTAAAPAGGA PPAHAPGPGR DAASAARGSR MRPHIFTLSV PFPTPLEAEI AHGSLAPDAE PHQRVVGKDL TVSGRILVVR WKAEDCRLLR ISVINFLDQL SLVVRTMQRF GPPVSR.

Product Science Overview

Structure and Expression

LAGE3 is ubiquitously expressed in many cell types and is often considered a notable up-regulated RNA modification-related protein in a majority of carcinoma cases . It is particularly significant in the context of hepatocellular carcinoma (HCC), where it has been identified as a potential prognostic biomarker and therapeutic target .

Functional Significance

LAGE3 maintains several important biological functions and has physiological significance within the CTAG family. It is involved in regulating the occurrence and invasion of numerous types of tumors . In HCC, LAGE3 is extensively expressed in cell lines such as BEL-7404, SMCC-7721, and Huh-7 cells, as well as in HCC tissues . However, lower expression levels are observed in HepG2 cells .

Mechanisms of Action

LAGE3 is implicated in several key signaling pathways that are crucial for cancer cell proliferation, migration, invasion, and apoptosis. Specifically, it has been shown to promote tumor development in HCC via the PI3K/AKT/mTOR and Ras/RAF/MAPK pathways . These pathways are essential for cell growth and survival, and their dysregulation is a common feature in many cancers.

Clinical Implications

The expression level of LAGE3 in HCC tissues is significantly higher compared to normal tissues, and high expression of LAGE3 is associated with a worse prognosis . Knocking down LAGE3 expression in HCC cell lines has been shown to increase apoptosis, inhibit growth rate, and reduce the progression of HCC in vivo . These findings suggest that LAGE3 could serve as an oncogenic factor and a potential therapeutic target for HCC .

Research and Future Directions

Ongoing research aims to further elucidate the functional and regulatory mechanisms of LAGE3 in cancer progression. The development of specific LAGE3-targeted drugs may offer new effective treatment modalities for patients with HCC . Additionally, bioinformatics analyses based on TCGA databases have revealed that recombinant human LAGE3 might function as an effective prognostic and diagnostic biomarker for HCC .

In conclusion, LAGE3 is a critical protein with significant implications in cancer biology, particularly in hepatocellular carcinoma. Its role as a prognostic biomarker and potential therapeutic target highlights the importance of continued research in this area.

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