KRT8 Antibody

Cytokeratin 8, Mouse Anti Human
Cat. No.
BT23626
Source
Synonyms
Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Cytokeratin 8 (KRT8), a member of the type II keratin family located on chromosome 12's long arm, plays a crucial role in epithelial cell structure and function. KRT8 partners with keratin 18 to create intermediate filaments, which are essential for maintaining cell shape and integrity in simple epithelial tissues. Beyond its structural role, KRT8 is implicated in cellular signaling pathways and differentiation processes. Mutations in the KRT8 gene have been linked to the development of cryptogenic cirrhosis.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody solution has a concentration of 1 mg/ml and is prepared in a buffer containing PBS at a pH of 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For long-term storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has undergone rigorous testing in both ELISA and Western blot applications, confirming its specificity and reactivity. While the recommended starting dilution is 1:1000, optimal working dilutions may vary depending on the specific application and should be determined through titration.
Synonyms
Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2.
Type
Mouse Anti Human Monoclonal.
Clone
PAT3D11A.
Immunogen
Anti-human KRT8 mAb, clone PAT3D11A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT8 protein purified from E. coli.
Ig Subclass
Mouse IgG3 heavy chain and Kappa light chain.

Product Science Overview

Introduction

Cytokeratin 8 (CK8) is a type II intermediate filament protein that plays a crucial role in the structural integrity and function of epithelial cells. It is often studied in the context of various diseases and cellular processes. The mouse anti-human Cytokeratin 8 antibody is a valuable tool in biomedical research for detecting and studying CK8 in human tissues.

Cytokeratin 8 Overview

Cytokeratin 8 is a member of the type II keratin family, which is clustered on the long arm of chromosome 12. It typically dimerizes with Cytokeratin 18 (CK18) to form intermediate filaments in simple single-layered epithelial cells . These filaments are essential for maintaining cellular structural integrity, signal transduction, and cellular differentiation . Mutations in the CK8 gene have been linked to various diseases, including cryptogenic cirrhosis .

Applications of Mouse Anti-Human Cytokeratin 8 Antibody

The mouse anti-human Cytokeratin 8 antibody is widely used in several scientific applications, including:

  • Immunohistochemistry (IHC): This technique involves staining tissue sections to visualize the presence and distribution of CK8 in different cell types and tissues .
  • Western Blot (WB): This method is used to detect and quantify CK8 protein levels in cell and tissue lysates .
  • Immunocytochemistry (ICC): Similar to IHC, ICC is used to stain and visualize CK8 in cultured cells .
  • Flow Cytometry: This technique allows for the analysis of CK8 expression in individual cells within a mixed population .
  • Enzyme-Linked Immunosorbent Assay (ELISA): ELISA is used to measure CK8 levels in various biological samples .
Specificity and Validation

The mouse anti-human Cytokeratin 8 antibody has been validated for specificity through various methods, including knockout, knockdown, and cell treatment experiments . This ensures that the antibody specifically binds to CK8 without cross-reacting with other proteins.

Research and Clinical Significance

Research involving CK8 and its antibody has provided valuable insights into the role of intermediate filaments in cellular processes and disease mechanisms. For example, studies have shown that CK8 is involved in maintaining the structural integrity of epithelial cells and plays a role in signal transduction and cellular differentiation . Additionally, mutations in the CK8 gene have been linked to cryptogenic cirrhosis, highlighting its clinical significance .

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