KPNA2 Human

Karyopherin Alpha 2 Human Recombinant
Cat. No.
BT25747
Source
E.coli.
Synonyms
Karyopherin subunit alpha 2 (RAG cohort 1 importin alpha 1), RCH1, QIP2, IPOA1, SRP1 alpha, RAG cohort protein 1, importin alpha 1, importin subunit alpha-2, importin-alpha-P1, pendulin.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KPNA2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 553 amino acids (1-529) and having a molecular mass of 60.5kDa.
KPNA2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
KPNA2, a member of the karyopherin alpha family, plays a crucial role in transporting proteins containing a conventional nuclear localization signal (NLS) into the nucleus. Studies have shown that KPNA2 directly binds to substrates with simple or bipartite NLS motifs, facilitating their interaction with the nuclear envelope. This interaction, in conjunction with Ran, enables the complete import of proteins into the nucleus.
Description
Recombinant KPNA2 Human, produced in E.coli, is a single, non-glycosylated polypeptide chain consisting of 553 amino acids (residues 1-529). With a molecular weight of 60.5kDa, this protein features a 24 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The KPNA2 solution is provided at a concentration of 1mg/ml in a buffer containing 20mM Tris-HCl (pH 8.0), 1mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein such as 0.1% HSA or BSA can further enhance stability during long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Karyopherin subunit alpha 2 (RAG cohort 1 importin alpha 1), RCH1, QIP2, IPOA1, SRP1 alpha, RAG cohort protein 1, importin alpha 1, importin subunit alpha-2, importin-alpha-P1, pendulin.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSTNEN ANTPAARLHR FKNKGKDSTE MRRRRIEVNV ELRKAKKDDQ MLKRRNVSSF PDDATSPLQE NRNNQGTVNW SVDDIVKGIN SSNVENQLQA TQAARKLLSR EKQPPIDNII RAGLIPKFVS FLGRTDCSPI QFESAWALTN IASGTSEQTK VVVDGGAIPA FISLLASPHA HISEQAVWAL GNIAGDGSVF RDLVIKYGAV DPLLALLAVP DMSSLACGYL RNLTWTLSNL CRNKNPAPPI DAVEQILPTL VRLLHHDDPE VLADTCWAIS YLTDGPNERI GMVVKTGVVP QLVKLLGASE LPIVTPALRA IGNIVTGTDE QTQVVIDAGA LAVFPSLLTN PKTNIQKEAT WTMSNITAGR QDQIQQVVNH GLVPFLVSVL SKADFKTQKE AVWAVTNYTS GGTVEQIVYL VHCGIIEPLM NLLTAKDTKI ILVILDAISN IFQAAENLGE TEKLSIMIEE CGGLDKIEAL QNHENESVYK ASLSLIEKYF SVEEEEDQNV VPETTSEGYT FQVQDGAPGT FNF.

Product Science Overview

Structure and Function

KPNA2 is a nuclear transporter that binds to nuclear localization signals (NLS) on cargo proteins, facilitating their transport into the nucleus. It forms a complex with karyopherin beta (importin beta), which mediates the translocation through the nuclear pore complex. Once inside the nucleus, the complex dissociates, releasing the cargo protein to perform its function.

Role in Carcinogenesis

KPNA2 has been implicated in the development and progression of various cancers. Its overexpression has been observed in several malignancies, including colon cancer, lung adenocarcinoma, and ovarian cancer . In colon cancer, KPNA2 expression is significantly higher in tumor tissues compared to normal tissues, and its expression correlates with advanced disease stages and poor prognosis . Similarly, in lung adenocarcinoma, high KPNA2 expression is associated with inferior overall survival . In ovarian cancer, increased KPNA2 expression predicts unfavorable prognosis .

Clinical Significance

The dysregulated expression of KPNA2 in cancers suggests its potential as a prognostic marker and therapeutic target. In colon cancer, KPNA2 has been shown to be an independent prognostic indicator of disease-free survival and overall survival . Knockdown of KPNA2 expression inhibits cancer cell proliferation, colony formation, and migration, highlighting its role in tumorigenesis . In lung adenocarcinoma, a prognostic model incorporating KPNA2 expression accurately predicts survival outcomes, providing potential targets for precision therapy .

Research and Therapeutic Potential

Ongoing research aims to further elucidate the role of KPNA2 in cancer and explore its potential as a therapeutic target. The development of inhibitors targeting KPNA2-mediated nuclear import could provide a novel approach for cancer treatment. Additionally, understanding the mechanisms underlying KPNA2’s role in immune homeostasis and tumor biology could lead to new insights into cancer pathogenesis and therapy.

In conclusion, KPNA2 is a critical player in nuclear transport and has significant implications in cancer biology. Its role as a prognostic marker and potential therapeutic target makes it a promising focus for future research and clinical applications.

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Source
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