KLRG1 Human

Killer Cell Lectin-like Receptor Subfamily G, Member 1 Human Recombinant
Cat. No.
BT219
Source
Escherichia Coli.
Synonyms
Killer cell lectin-like receptor subfamily G member 1, C-type lectin domain family 15 member A, ITIM-containing receptor MAFA-L, MAFA-like receptor, Mast cell function-associated antigen, KLRG1, CLEC15A, MAFA, MAFAL, 2F1, MAFA-L, MAFA-2F1, MAFA-LIKE.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KLRG1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids (60-189 a.a) and having a molecular mass of 17kDa.
KLRG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
KLRG1, also known as Killer Cell Lectin-like Receptor Subfamily G, Member 1, belongs to the killer cell lectin-like receptor (KLR) family. This family comprises transmembrane proteins primarily found on NK cells. Natural killer (NK) cells are lymphocytes capable of directly killing specific tumor cells and virus-infected cells without prior activation. They also play a regulatory role in humoral and cell-mediated immune responses. Notably, the expression of KLRG1 on the cell surface is enhanced by the presence of MHC class I molecules.
Description
This product consists of a recombinant human KLRG1 protein produced in E. coli. It is a single, non-glycosylated polypeptide chain with 153 amino acids (residues 60-189). This protein has a molecular weight of 17 kDa and includes a 23 amino acid His-tag fused at its N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The KLRG1 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freezing and thawing should be avoided.
Purity
The purity of this product is greater than 85.0%, as assessed by SDS-PAGE.
Synonyms
Killer cell lectin-like receptor subfamily G member 1, C-type lectin domain family 15 member A, ITIM-containing receptor MAFA-L, MAFA-like receptor, Mast cell function-associated antigen, KLRG1, CLEC15A, MAFA, MAFAL, 2F1, MAFA-L, MAFA-2F1, MAFA-LIKE.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSLCQGSNY STCASCPSCP DRWMKYGNHC YYFSVEEKDW NSSLEFCLAR DSHLLVITDN QEMSLLQVFL SEAFCWIGLR NNSGWRWEDG SPLNFSRISS NSFVQTCGAI NKNGLQASSC EVPLHWVCKK VRL.

Product Science Overview

Introduction

Killer Cell Lectin-like Receptor Subfamily G, Member 1 (KLRG1) is a type II transmembrane protein that plays a crucial role in the immune system. It is predominantly expressed on natural killer (NK) cells and certain T cells, where it functions as an inhibitory receptor. The human recombinant form of KLRG1 is a synthesized version of this protein, used in various research and therapeutic applications.

Gene and Protein Structure

The KLRG1 gene is located on chromosome 12 in humans and encodes a protein that belongs to the killer cell lectin-like receptor (KLR) family . This family comprises transmembrane proteins that are primarily expressed in NK cells. The KLRG1 protein has a lectin-like domain that allows it to bind to specific carbohydrate structures on target cells .

Expression and Regulation

KLRG1 is expressed on late-differentiated effector and effector memory CD8+ T cells and NK cells . Its expression is regulated by major histocompatibility complex (MHC) class I molecules, which are essential for the immune system’s ability to recognize and respond to pathogens .

Function

KLRG1 functions as an inhibitory receptor, playing a critical role in regulating the effector functions and developmental processes of NK cells and T cells . It binds to non-MHC ligands such as E-cadherin, N-cadherin, and R-cadherin, which are markers of epithelial and mesenchymal cells . This binding inhibits NK cell-mediated cytotoxicity and interferon-gamma secretion, thereby modulating the immune response .

Clinical Significance

The role of KLRG1 in immune regulation has significant implications for various diseases, including cancer and infectious diseases. Targeting KLRG1 and other co-inhibitory receptors has gained interest in oncology for enhancing immune activation against tumors . Additionally, KLRG1 knockout mice have shown enhanced immunity in certain disease models, highlighting its potential as a therapeutic target .

Applications of Human Recombinant KLRG1

The human recombinant form of KLRG1 is used in research to study its function and interactions with ligands. It is also utilized in developing therapeutic strategies aimed at modulating immune responses. By understanding the mechanisms of KLRG1, researchers can design interventions to enhance or inhibit its activity, depending on the desired outcome.

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Source
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