KLRC2 Human

Killer Cell Lectin-Like Receptor Subfamily C, Member 2 Human Recombinant
Cat. No.
BT27107
Source
E.coli.
Synonyms
Killer cell lectin-like receptor subfamily C member 2, NKG2-C type II integral membrane protein, NKG2-C-activating NK receptor, CD159 antigen-like family member C, NK cell receptor C, NKG2C, CD159c.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KLRC2 Human Recombinant produced in E. coli is a single polypeptide chain containing 162 amino acids (94-231) and having a molecular mass of 18.4 kDa.
KLRC2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The KLRC2 gene encodes a receptor for MHC class I HLA-E molecules, primarily expressed on natural killer (NK) cells and some cytotoxic T-cells. KLRC2 belongs to the NKG2 (KLRC) family, a group of transmembrane proteins within the NK complex known for their type II membrane orientation and C-type lectin domain. These proteins play a crucial role in immune recognition. While multiple KLRC2 splice variants exist, their complete characterization remains to be fully elucidated.
Description
Recombinant human KLRC2, expressed in E. coli, is a single polypeptide chain with a molecular weight of 18.4 kDa. This protein comprises 162 amino acids (residues 94-231) and includes a 24 amino acid His-tag fused at its N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
KLRC2 is supplied as a 0.5 mg/mL solution in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 85% based on SDS-PAGE analysis.
Synonyms
Killer cell lectin-like receptor subfamily C member 2, NKG2-C type II integral membrane protein, NKG2-C-activating NK receptor, CD159 antigen-like family member C, NK cell receptor C, NKG2C, CD159c.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMIPFLEQ NNFSPNTRTQ KARHCGHCPE EWITYSNSCY YIGKERRTWE ESLLACTSKN SSLLSIDNEE EMKFLASILP SSWIGVFRNS SHHPWVTING LAFKHKIKDS DNAELNCAVL QVNRLKSAQC GSSMIYHCKH KL

Product Science Overview

Introduction

Killer Cell Lectin-Like Receptor Subfamily C, Member 2 (KLRC2), also known as NKG2C or CD159c, is a protein encoded by the KLRC2 gene in humans. This protein is primarily expressed on natural killer (NK) cells, which are a type of lymphocyte involved in the innate immune response. The recombinant form of this protein is often used in research to study its function and interactions.

Gene and Protein Structure

The KLRC2 gene is located on chromosome 12 (12p13.2) and encodes a type II transmembrane protein characterized by an extracellular C-terminus and a C-type lectin domain . The protein is part of the NK complex, a region containing several C-type lectin genes preferentially expressed on NK cells .

Function

KLRC2 plays a crucial role in the immune system by mediating the lysis of certain tumor cells and virus-infected cells without prior activation . It also regulates specific humoral and cell-mediated immunity. The protein forms heterodimers with CD94, another NK cell receptor, and this complex can bind to HLA-E, a non-classical MHC class I molecule . This binding leads to the activation of NK cells, particularly during infections with human cytomegalovirus (HCMV), where viral peptides are presented on HLA-E .

Pathways and Interactions

KLRC2 is involved in several immune pathways, including DAP12 interactions and the innate immune system . The CD94/NKG2C heterodimer specifically recognizes viral peptides presented by HLA-E, leading to the activation, expansion, and differentiation of adaptive NK cells . This interaction is crucial for the immune response against HCMV and other viral infections.

Clinical Significance

Mutations or dysregulation of KLRC2 have been associated with various diseases, including Hyper IgE Recurrent Infection Syndrome 2 and Peroxisome Biogenesis Disorder 1A . Understanding the function and regulation of KLRC2 is essential for developing therapeutic strategies for these conditions.

Research Applications

The recombinant form of KLRC2 is widely used in research to study its structure, function, and interactions. It is also used in the development of therapeutic agents targeting NK cell-mediated immunity.

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