KLRB1 Human, Sf9
Sf9, Baculovirus cells.
Killer Cell Lectin Like Receptor B1, Natural Killer Cell Surface Protein P1A, Killer Cell Lectin-Like Receptor Subfamily B, Member 1, C-Type Lectin Domain Family 5 Member B, HNKR-P1A, NKR-P1A, CLEC5B, NKRP1A, Killer Cell Lectin-Like Receptor Subfamily B Member 1, CD161 Antigen, NKR-P1, CD161, NKR.
Greater than 90% as determined by SDS-PAGE.
Description
KLRB1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 401 amino acids (67-225a.a.) and having a molecular mass of 45.7kDa.
KLRB1 is expressed with a 239 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Killer Cell Lectin Like Receptor B1, Natural Killer Cell Surface Protein P1A, Killer Cell Lectin-Like Receptor Subfamily B, Member 1, C-Type Lectin Domain Family 5 Member B, HNKR-P1A, NKR-P1A, CLEC5B, NKRP1A, Killer Cell Lectin-Like Receptor Subfamily B Member 1, CD161 Antigen, NKR-P1, CD161, NKR.
Sf9, Baculovirus cells.
ADPQKSSIEK CSVDIQQSRN KTTERPGLLN CPIYWQQLRE KCLLFSHTVN PWNNSLADCS TKESSLLLIR DKDELIHTQN LIRDKAILFW IGLNFSLSEK NWKWINGSFL NSNDLEIRGD AKENSCISIS QTSVYSEYCS TEIRWICQKE LTPVRNKVYP DSLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H.
Product Science Overview
Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1), also known as CD161, is a protein encoded by the KLRB1 gene in humans. This protein plays a crucial role in the immune system, particularly in the function of natural killer (NK) cells and certain T cells. The recombinant form of this protein, produced in Sf9 insect cells, is used in various research and therapeutic applications.
The KLRB1 gene is located on chromosome 12p13.31 in humans . The protein encoded by this gene is a type II transmembrane protein, meaning it has an external C terminus. It contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain . The recombinant form produced in Sf9 cells is a single, glycosylated polypeptide chain containing 401 amino acids and has a molecular mass of 45.7 kDa .
KLRB1 is primarily expressed on the surface of NK cells and certain T cells. It acts as an inhibitory receptor, playing a significant role in regulating the cytotoxic activity of NK cells. The protein binds to specific ligands, such as CLEC2D/LLT1, which leads to the inhibition of NK cell-mediated cytotoxicity and interferon-gamma secretion . This regulatory function is crucial for maintaining immune homeostasis and preventing excessive immune responses.
The recombinant form of KLRB1 is produced using the baculovirus expression system in Sf9 insect cells. This system is widely used for producing recombinant proteins due to its ability to perform post-translational modifications similar to those in mammalian cells. The recombinant KLRB1 produced in Sf9 cells is tagged with a 239 amino acid hIgG-His-tag at the C-terminus and purified using proprietary chromatographic techniques .
Recombinant KLRB1 has several applications in research and therapeutic settings. It is used to study the mechanisms of NK cell regulation and to develop therapies targeting immune-related diseases. Additionally, it serves as a valuable tool in immunological assays and the development of diagnostic tests.
