KLK7 Human, sf9

Kallikrein-7 is a 253 amino acid protein consisting of a 22 amino acid signal peptide and a 7 amino acid propeptide . The recombinant form of Kallikrein-7 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 190 amino acids (1-181 a.a.) and has a molecular mass of 20.9 kDa . This recombinant protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .

Kallikrein-7 plays a crucial role in the proteolysis of intercellular cohesive structures preceding desquamation, which is the shedding of the outermost layer of the skin . It is specific for amino acid residues with aromatic side chains in the P1 position and is involved in the activation of precursors to inflammatory cytokines . This enzyme is expressed in skin cells and is essential for maintaining skin homeostasis.

Recombinant Human Kallikrein-7 is produced in Sf9 Baculovirus cells and purified via sequential chromatography . The activation of Kallikrein-7 involves a reaction containing 100 μg/mL Kallikrein-7, 10 μg/mL thermolysin, 50 mM Tris, CaCl2, 150 mM NaCl, and 0.05% Brij 35 at pH 7.5 for 2 hours at 37°C . The thermolysin reaction is stopped by adding EDTA to a final concentration of 50 mM .

Kallikrein-7 is used in various research applications, particularly in studies related to skin biology and inflammatory processes. The enzyme’s specific activity is greater than 300 pmoles/min/μg when measured with a fluorogenic peptide substrate . It is typically formulated as a lyophilized, carrier-free product and can be reconstituted in sterile PBS to a concentration of 0.1–0.5 mg/mL .

Lyophilized Human Kallikrein-7 should be stored at 2°C to 8°C, preferably desiccated . Reconstituted Kallikrein-7 should be stored at ≤ –20°C and kept freeze-thaw cycles to a minimum . For long-term storage, it is recommended to add a carrier protein such as 0.1% HSA or BSA .