Kallikrein-5 (KLK5) is a member of the serine protease family, which is a group of proteolytic enzymes. These enzymes are known for their ability to cleave peptide bonds in proteins, a function that is crucial in various physiological processes. KLK5 is also referred to by several other names, including Kallikrein-like protein 2 (KLK-L2) and Stratum corneum tryptic enzyme (SCTE) .
KLK5 is expressed in a variety of tissues, including the salivary gland, stomach, uterus, lung, thymus, prostate, colon, brain, thyroid, and trachea . Its expression is up-regulated by estrogens and progestins, indicating a hormonal regulation mechanism . This enzyme is secreted and plays a role in the desquamation process in the epidermis, which is the shedding of the outermost layer of the skin .
The recombinant form of KLK5, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 236 amino acids (67-293 a.a.) and has a molecular mass of approximately 26.2 kDa . The protein is expressed with a 6 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques . The physical appearance of the protein is a sterile, filtered colorless solution, and it is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol .
KLK5 protein solution should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it should be frozen at -20°C. To ensure long-term stability, it is recommended to add a carrier protein such as 0.1% Human Serum Albumin (HSA) or Bovine Serum Albumin (BSA). It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .