KLK2 is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. It is predominantly expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen (pro-PSA) into its enzymatically active form . This makes KLK2 particularly significant in the context of prostate health and disease.
The recombinant form of KLK2, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 246 amino acids (25-261 aa) and has a molecular mass of approximately 27.2 kDa. Under reducing conditions, it migrates at 28-40 kDa on SDS-PAGE . The recombinant protein is expressed with a 6-amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .
KLK2 is greatly expressed in prostate tumor cells and may serve as a prognostic marker for prostate cancer risk . Its role in activating pro-PSA highlights its importance in the regulation of prostate-specific antigen levels, which are commonly used as a biomarker for prostate cancer screening and diagnosis.
The production of recombinant KLK2 in Sf9 Baculovirus cells involves the use of a baculovirus expression system, which is known for its ability to produce high yields of recombinant proteins with post-translational modifications similar to those in mammalian cells. The recombinant KLK2 is formulated as a sterile, filtered, colorless solution containing phosphate-buffered saline (pH 7.4) and 10% glycerol .
For optimal stability, the KLK2 protein solution should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it is recommended to store the solution frozen at -20°C. To prevent degradation, it is advisable to add a carrier protein (0.1% HSA or BSA) and avoid multiple freeze-thaw cycles .
KLK2 has various applications in research, particularly in studies related to prostate cancer. Its ability to activate pro-PSA makes it a valuable tool for understanding the mechanisms underlying prostate cancer progression and for developing potential therapeutic strategies.