KLK13 Human, sf9

KLK13 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 . The gene expression of KLK13 is regulated by steroid hormones, making it a potential marker for hormone-related cancers such as breast cancer .

The recombinant form of KLK13 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 267 amino acids (17-277 a.a.) and has a molecular mass of approximately 29.7 kDa . The protein is expressed with a 6 amino acid His tag at the C-terminus, which facilitates its purification using chromatographic techniques .

KLK13 is produced in Sf9 Baculovirus cells, which are insect cells commonly used for the expression of recombinant proteins. The protein is purified to a high degree of purity (>90%) using proprietary chromatographic techniques . The final product is a sterile, filtered, colorless solution formulated in phosphate-buffered saline (pH 7.4) with 10% glycerol .

KLK13 exhibits specific enzymatic activity, with a specific activity greater than 8,000 pmol/min/µg. One unit of KLK13 will hydrolyze 1.0 pmole of Na-Benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 8.0 and 25°C . This activity is crucial for its role in various physiological processes and potential therapeutic applications.

For optimal stability, KLK13 should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it is recommended to store the protein frozen at -20°C. To prevent degradation, it is advisable to add a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) and avoid multiple freeze-thaw cycles .

Due to its regulated expression by steroid hormones and its potential role in carcinogenesis, KLK13 is being studied as a biomarker for breast cancer and other hormone-related cancers . Its enzymatic properties also make it a valuable tool for various biochemical and physiological studies.