KLK10 Human

Kallikrein-10 Human Recombinant
Cat. No.
BT3125
Source
Escherichia Coli.
Synonyms
Kallikrein-Related Peptidase 10, KLK10, PRSSL1, NES1, Normal Epithelial Cell-Specific 1, Protease Serine-Like 1, Kallikrein 10, Breast Normal Epithelial Cell Associated Serine Protease, kallikrein-10, Protease, Serine-Like 1, EC 3.4.21.- , EC 3.4.21, EC 3.4.21.74.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

KLK10 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 266 amino acids (34-276) and having a molecular mass of 29.1kDa.
KLK10 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Kallikrein-10, also known as KLK10, is a serine protease belonging to the kallikrein family. This family consists of 15 members found clustered on chromosome 19. KLK10 is involved in various physiological processes and plays a significant role in suppressing tumor development in breast and prostate cancers.
Description
Recombinant human KLK10, expressed in E.coli, is available as a single, non-glycosylated polypeptide chain. This protein encompasses 266 amino acids (residues 34-276) with a molecular weight of 29.1kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus. The purification process utilizes proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless solution that has been filtered.
Formulation
The KLK10 solution is provided at a concentration of 1mg/ml. The formulation buffer consists of 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep the solution at 4°C. For extended storage, freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
The purity of KLK10 is determined by SDS-PAGE analysis and exceeds 85%.
Synonyms
Kallikrein-Related Peptidase 10, KLK10, PRSSL1, NES1, Normal Epithelial Cell-Specific 1, Protease Serine-Like 1, Kallikrein 10, Breast Normal Epithelial Cell Associated Serine Protease, kallikrein-10, Protease, Serine-Like 1, EC 3.4.21.- , EC 3.4.21, EC 3.4.21.74.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSALLPQND TRLDPEAYGS PCARGSQPWQ VSLFNGLSFH CAGVLVDQSW VLTAAHCGNK PLWARVGDDH LLLLQGEQLR RTTRSVVHPK YHQGSGPILP RRTDEHDLML LKLARPVVLG PRVRALQLPY RCAQPGDQCQ VAGWGTTAAR RVKYNKGLTC SSITILSPKE CEVFYPGVVT NNMICAGLDR GQDPCQSDSG GPLVCDETLQ GILSWGVYPC GSAQHPAVYT QICKYMSWIN KVIRSN.

Product Science Overview

Introduction

Kallikrein-10 (KLK10), also known as normal epithelial cell-specific 1 (NES1), is a member of the human kallikrein gene family. This family consists of 15 genes located on chromosome 19q13.3-q13.4, which encode for serine proteases involved in various physiological processes .

Gene and Protein Structure

The KLK10 gene encodes for a secreted serine protease known as human kallikrein 10 (hK10). This protein is expressed in various tissues, including the salivary glands, skin, and colon. It is also detectable in biological fluids such as breast milk, seminal plasma, cerebrospinal fluid, amniotic fluid, and serum .

Biological Functions

hK10 is hypothesized to play a role in several physiological processes, including tissue remodeling, cell migration, and immune response. Its concentration changes in some disease states, suggesting its potential as a biomarker for certain conditions .

Recombinant Production

Recombinant hK10 protein is produced and purified using a Pichia pastoris yeast expression system. This recombinant protein is used as an immunogen to generate mouse and rabbit polyclonal anti-hK10 antisera. These antibodies are then utilized to develop a sensitive and specific immunoassay for hK10 .

Clinical Significance

The development of the first immunofluorometric assay for hK10 has enabled researchers to study its distribution in biological fluids and tissue extracts. This assay has a detection limit of 0.05 μg/L and is specific for hK10, with no detectable cross-reactivity with other homologous kallikrein proteins . The assay’s sensitivity and specificity make it a valuable tool for examining the potential of hK10 as a disease biomarker.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

KLK10 Human, Sf9
Kallikrein-10 Human Recombinant, Sf9
Cat. No.
BT2862
Source
<p>Sf9, Baculovirus cells.</p>
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.