KIT Human

KIT Proto-Oncogene Receptor Tyrosine Human Recombinant
Cat. No.
BT11062
Source

Sf9, Baculovirus.

Synonyms

Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KIT produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 507 amino acids (26-524 a.a.) and having a molecular mass of 57.1kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
KIT is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
KIT, also known as KIT Proto-Oncogene Receptor Tyrosine, is a cytokine receptor expressed in hematopoietic stem cells and other cell types. KIT binds to receptor tyrosine kinase type III, a stem cell factor also called "rigid factor" or "c-kit ligand." Upon binding to stem cell factor (SCF), the receptor dimerizes, activating intrinsic tyrosine kinase activity. This activation triggers the phosphorylation and activation of signaling molecules, generating cellular signals. This receptor plays a role in protecting vascular smooth muscle cells from apoptosis and restoring cardiac function after myocardial infarction.
Description
Produced in Sf9 Insect cells, KIT is a single, glycosylated polypeptide chain consisting of 507 amino acids (26-524 a.a.). It has a molecular mass of 57.1kDa, though it appears as approximately 50-70kDa on SDS-PAGE. The protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The KIT protein solution has a concentration of 0.25mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For use within 2-4 weeks, store at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 90.0% as determined by SDS-PAGE.
Synonyms

Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117.

Source

Sf9, Baculovirus.

Amino Acid Sequence

QPSVSPGEPS PPSIHPGKSD LIVRVGDEIR LLCTDPGFVK WTFEILDETN ENKQNEWITE KAEATNTGKY TCTNKHGLSN SIYVFVRDPA KLFLVDRSLY GKEDNDTLVR CPLTDPEVTN YSLKGCQGKP LPKDLRFIPD PKAGIMIKSV KRAYHRLCLH CSVDQEGKSV LSEKFILKVR PAFKAVPVVS VSKASYLLRE GEEFTVTCTI KDVSSSVYST WKRENSQTKL QEKYNSWHHG DFNYERQATL TISSARVNDS GVFMCYANNT FGSANVTTTL EVVDKGFINI FPMINTTVFV NDGENVDLIV EYEAFPKPEH QQWIYMNRTF TDKWEDYPKS ENESNIRYVS ELHLTRLKGT
EGGTYTFLVS NSDVNAAIAF NVYVNTKPEI LTYDRLVNGM LQCVAAGFPE PTIDWYFCPG TEQRCSASVL PVDVQTLNSS GPPFGKLVVQ SSIDSSAFKH NGTVECKAYN DVGKTSAYFN FAFKGNNKEQ IHPHTLFTPL EHHHHHH.

Product Science Overview

Introduction

The KIT proto-oncogene receptor tyrosine kinase, also known as CD117 or c-Kit, is a protein encoded by the KIT gene in humans. This receptor tyrosine kinase plays a crucial role in various cellular processes, including cell survival, proliferation, and differentiation .

Discovery and Structure

The KIT gene was first described by the German biochemist Axel Ullrich in 1987 as the cellular homolog of the feline sarcoma viral oncogene v-kit . The KIT protein consists of several domains:

  • Extracellular domain: Composed of five immunoglobulin-like domains.
  • Transmembrane domain: Anchors the receptor in the cell membrane.
  • Juxtamembrane domain: Located just inside the cell membrane.
  • Intracellular tyrosine kinase domain: Responsible for the receptor’s kinase activity .
Function

KIT is a cytokine receptor expressed on the surface of hematopoietic stem cells and other cell types. It binds to its ligand, the stem cell factor (SCF), also known as “steel factor” or "c-kit ligand" . Upon binding to SCF, KIT forms a dimer, activating its intrinsic tyrosine kinase activity. This activation leads to the phosphorylation of various signal transduction molecules, propagating the signal within the cell .

Role in Cellular Processes

KIT signaling is essential for several cellular processes:

  • Hematopoiesis: The formation of blood cellular components.
  • Melanogenesis: The production of melanin in melanocytes.
  • Gametogenesis: The development of gametes (sperm and eggs).
  • Mast cell development: Involves the migration and function of mast cells .
Clinical Significance

Alterations in the KIT gene or its expression can lead to various diseases, including:

  • Gastrointestinal stromal tumors (GISTs): KIT mutations are commonly associated with these tumors.
  • Mast cell disease: Abnormal KIT signaling can lead to mastocytosis.
  • Acute myelogenous leukemia (AML): KIT mutations are found in some cases of AML.
  • Piebaldism: A genetic disorder characterized by the absence of melanocytes in certain areas of the skin and hair .
Therapeutic Implications

Targeted therapies have been developed to inhibit KIT activity in diseases where it is overactive. Drugs like nilotinib and sunitinib have shown efficacy in treating patients with KIT overactivity, particularly in GISTs and melanomas .

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