KISS1 Human
Description
The KISS1 is purified by proprietary chromatographic techniques.
Product Specs
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Product Science Overview
The KISS-1 gene was first identified as a metastasis-suppressor gene in human malignant melanoma cells. Researchers discovered that the introduction of human chromosome 6 into metastatic melanoma cells significantly suppressed their ability to metastasize without affecting their tumorigenicity . This led to the isolation of a novel complementary DNA (cDNA) from these cells, which was designated as KISS-1 .
KISS-1 encodes a protein that is predominantly hydrophilic and consists of 164 amino acids. This protein includes a polyproline-rich domain, indicative of an SH3 ligand, and a putative protein kinase C-α phosphorylation site . The expression of KISS-1 mRNA is observed in nonmetastatic melanoma cells, while it is undetectable in normal tissues such as the heart, brain, liver, lung, and skeletal muscle. However, weak expression is found in the kidney and pancreas, with the highest expression observed in the placenta .
KISS-1 functions by inhibiting chemotaxis and invasion, thereby attenuating metastasis in malignant melanomas . It is involved in the regulation of cell-matrix adhesion and plays a role in cytoskeletal reorganization . The metastasis-suppressor properties of KISS-1 may be mediated in part by cell cycle arrest and the induction of apoptosis in malignant cells .
The expression of KISS-1 has significant clinical implications. It may serve as a useful marker for distinguishing metastatic melanomas from nonmetastatic melanomas . Additionally, the transfection of full-length KISS-1 cDNA into melanoma cells has been shown to suppress metastasis in an expression-dependent manner . This suggests that KISS-1 could potentially be used as a therapeutic target for preventing metastasis in malignant melanomas and other cancers.
Recombinant KISS-1 protein is produced using recombinant DNA technology, which involves inserting the KISS-1 gene into a suitable expression system to produce the protein in large quantities. This recombinant protein can be used in various research applications to study its role in metastasis suppression and to develop potential therapeutic strategies .
