KIAA0513 Human

KIAA0513 Human Recombinant
Cat. No.
BT18854
Source
Escherichia Coli.
Synonyms
Uncharacterized protein KIAA0513 isoform b.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KIAA0513 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 424 amino acids (1-401 a.a) and having a molecular mass of 47.9kDa.
KIAA0513 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
KIAA0513 is a protein primarily found in the human brain, with significant presence in regions like the cortex, cerebellum, and hippocampus. Its expression is observed in both neurons and glial cells. Studies have indicated elevated levels of KIAA0513 in the dorsolateral prefrontal cortex of individuals diagnosed with schizophrenia compared to those without the condition.
Description
This product consists of a recombinant human KIAA0513 protein produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain composed of 424 amino acids (specifically, amino acids 1 to 401), resulting in a molecular weight of 47.9 kDa. The protein includes a 23 amino acid His-tag attached to its N-terminus to facilitate purification, which is carried out using proprietary chromatographic methods.
Physical Appearance
The product appears as a clear solution that has undergone sterile filtration.
Formulation
The KIAA0513 protein is provided at a concentration of 0.25 mg/ml in a solution of phosphate-buffered saline (pH 7.4) containing 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, freezing at -20°C is recommended. To further enhance stability during long-term storage, the addition of a carrier protein like HSA or BSA (0.1%) is advised. It is important to minimize repeated freezing and thawing of the product.
Purity
Analysis by SDS-PAGE indicates a purity level exceeding 85.0%.
Synonyms
Uncharacterized protein KIAA0513 isoform b.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMETPEVP VGSLIDFGPE APTSSPLEAP PPVLQDGDGS LGDGASESET TESADSENDM GESPSHPSWD QDRRSSSNES FSSNQSTEST QDEETLALRD FMRGYVEKIF SGGEDLDQEE KAKFGEYCSS ENGKGREWFA RYVSAQRCNS KCVSEATFYR LVQSFAVVLF ECHQMDDFGP AKNLMTMCFT YYHIGKPQLL PPESREKPAG SIDSYLKSAN SWLAEKKDIA ERLLKNTSAR TENVKGFFGG LETKLKGPLA RRNEEDENKP QEKRPRAVTA YSPEDEKKGE KIYLYTHLKQ QPIWHTLRFW NAAFFDAVHC ERTKRSPTTR EKWCHMTQEE RDDSLRFNEN ITFGQLGTFT HNMLAFGLNK KLCNDFLKKQ AVIGNLDEEQ YKLLSDHIEQ MATE.

Product Science Overview

Recombinant KIAA0513 Protein

Recombinant KIAA0513 protein is a human full-length protein that is often used in research to study its function and potential role in diseases. The recombinant version of this protein is typically produced in Escherichia coli (E. coli), a common bacterial expression system used for producing recombinant proteins .

Production and Purification

The production of recombinant KIAA0513 involves cloning the KIAA0513 gene into an expression vector, which is then introduced into E. coli cells. These cells are cultured under conditions that promote the expression of the KIAA0513 protein. Once expressed, the protein is purified using various chromatographic techniques to achieve high purity levels, often greater than 85% .

Applications

Recombinant KIAA0513 protein is used in various applications, including:

  • SDS-PAGE: A technique used to separate proteins based on their molecular weight.
  • Mass Spectrometry (MS): Used to analyze the protein’s structure and composition.
  • Western Blotting: A method used to detect specific proteins in a sample.
  • ELISA (Enzyme-Linked Immunosorbent Assay): Used to measure the concentration of the protein in a sample .
Research and Clinical Implications

Research on KIAA0513 is ongoing, and scientists are working to uncover its exact role in the human body. Understanding the function of KIAA0513 could have significant implications for the treatment of neurological disorders and other diseases.

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THE BIOTEK
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