KIAA0101 Human

KIAA0101 Human Recombinant
Cat. No.
BT18746
Source
Escherichia Coli.
Synonyms
KIAA0101, L5, NS5ATP9, OEATC, OEATC-1, OEATC1, p15(PAF), p15/PAF, p15PAF, PAF, PAF15, PCNA-associated factor, Hepatitis C virus NS5A-transactivated protein 9, HCV NS5A-transactivated protein 9, Overexpressed in anaplastic thyroid carcinoma 1, PCNA-associated factor of 15 kDa.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

KIAA0101 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 134 amino acids (1-111) and having a molecular mass of 14.4 kDa.
KIAA0101 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
KIAA0101, a PCNA-binding protein, plays a crucial role in regulating DNA repair during DNA replication. Upon DNA damage, its interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion synthesis DNA polymerase eta (POLH) at stalled replication forks. This interaction aids in bypassing lesions that obstruct replication fork progression. Additionally, KIAA0101 acts as a regulator of centrosome numbers.
Description
Recombinant human KIAA0101, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 134 amino acids (residues 1-111) with a molecular weight of 14.4 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The KIAA0101 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the protein in frozen aliquots at -20°C. To ensure long-term stability, adding a carrier protein such as 0.1% HSA or BSA is advisable. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of the protein is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
KIAA0101, L5, NS5ATP9, OEATC, OEATC-1, OEATC1, p15(PAF), p15/PAF, p15PAF, PAF, PAF15, PCNA-associated factor, Hepatitis C virus NS5A-transactivated protein 9, HCV NS5A-transactivated protein 9, Overexpressed in anaplastic thyroid carcinoma 1, PCNA-associated factor of 15 kDa.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVRTKAD SVPGTYRKVV AARAPRKVLG SSTSATNSTS VSSRKAENKY AGGNPVCVRP TPKWQKGIGE FFRLSPKDSE KENQIPEEAG SSGLGKAKRK ACPLQPDHTN DEKE.

Product Science Overview

Structure and Expression

The recombinant human KIAA0101 protein is typically expressed in Escherichia coli (E. coli) and consists of 126 amino acids with a calculated molecular mass of 13.8 kDa. When analyzed under reducing conditions using SDS-PAGE, it migrates as an approximately 19 kDa band . The protein is often tagged with a polyhistidine (His) tag at the N-terminus to facilitate purification .

KIAA0101 is predominantly localized in the nucleus and shares a conserved PCNA binding motif with several other PCNA binding proteins, including the CDK inhibitor p21 . It is expressed mainly in the liver, pancreas, and placenta, but is not detectable in the heart or brain .

Biological Function

KIAA0101 plays a crucial role in cell proliferation and has been implicated in early tumor recurrence (ETR) and the prognosis of hepatocellular carcinoma (HCC) . Its interaction with PCNA suggests that it may be involved in DNA replication and repair processes, which are essential for maintaining genomic stability during cell division.

Applications

Recombinant human KIAA0101 protein is used in various research applications, including studies on cell cycle regulation, cancer biology, and protein-protein interactions. Its high purity and stability make it suitable for use in biochemical assays, structural biology, and other experimental setups .

Storage and Handling

Recombinant KIAA0101 protein is typically provided as a lyophilized powder and should be stored under sterile conditions at -20°C to -80°C. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles, which can affect its stability and activity .

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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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