KCNIP3 Human
Kv Channel Interacting Protein 3 Human Recombinant
Cat. No.
BT17722
Source
Escherichia Coli.
Synonyms
Calsenilin, A-type potassium channel modulatory protein 3, DRE-antagonist modulator, Kv channel-interacting protein 3, KCNIP3, CSEN, DREAM, KCHIP3, MGC18289.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
KCNIP3 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 276 amino acids (1-256 a.a.) and having a molecular mass of 31.4kDa. The KCNIP3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
KCNIP3, a member of the voltage-gated potassium (Kv) channel-interacting protein family, belongs to the recoverin branch of the EF-hand superfamily. This family comprises small calcium-binding proteins with EF-hand-like domains. KCNIP3 acts as a calcium-regulated transcriptional repressor and interacts with presenilins. Mutations in presenilin genes have been linked to Alzheimer's disease.
Description
Recombinant human KCNIP3, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein, fused with a 20 amino acid His tag at its N-terminus, consists of 276 amino acids (residues 1-256) and has a molecular weight of 31.4 kDa. Purification of KCNIP3 is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile, colorless solution, filtered for purity.
Formulation
The KCNIP3 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, 2mM DTT, and 0.1M NaCl.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing cycles should be avoided.
Purity
Purity exceeds 90.0%, as determined by SDS-PAGE analysis.
Synonyms
Calsenilin, A-type potassium channel modulatory protein 3, DRE-antagonist modulator, Kv channel-interacting protein 3, KCNIP3, CSEN, DREAM, KCHIP3, MGC18289.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MQPAKEVTKA SDGSLLGDLG HTPLSKKEGI KWQRPRLSRQ ALMRCCLVKW ILSSTAPQGS DSSDSELELS TVRHQPEGLD QLQAQTKFTK KELQSLYRGF KNECPTGLVD EDTFKLIYAQ FFPQGDATTY AHFLFNAFDA DGNGAIHFED FVVGLSILLR GTVHEKLKWA FNLYDINKDG YITKEEMLAI MKSIYDMMGR HTYPILREDA PAEHVERFFE KMDRNQDGVV TIEEFLEACQ KDENIMSSMQ LFENVI.
Product Science Overview
Structural Properties
KChIP3 is encoded by the KCNIP3 gene and shares high sequence similarity with other KChIP family members, including KChIP1, KChIP2, and KChIP4 . The protein consists of a conserved C-terminal core domain and an N-terminal region that undergoes diverse post-translational modifications, determining its subcellular localization .
Expression Patterns and Tissue Distribution
Biological Functions
KChIP3 serves multiple biological functions:
- Regulation of Kv4 Channels: KChIP3 binds to the N-terminus of Kv4 channel α-subunits, promoting their translocation to the cell membrane, accelerating voltage-dependent activation, and slowing the recovery rate of inactivation .
- Transcriptional Repression: KChIP3 acts as a calcium-regulated transcriptional repressor by binding to the downstream regulatory element (DRE) on the promoters of target genes . This function is implicated in the regulation of pain, memory, endocrine, immune, and inflammatory reactions .
- Interaction with Presenilins: KChIP3 interacts with presenilins, which are proteins implicated in Alzheimer’s disease .
Modes of Action
Regulatory Mechanisms
Pathological Roles
Altered expression of KChIP3 has been implicated in several neurological and cardiovascular diseases:
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