KAT2A Antibody
Histone acetyltransferase KAT2A, Mouse Anti Human
Cat. No.
BT20420
Source
Synonyms
Histone acetyltransferase KAT2A, General control of amino acid synthesis protein 5-like 2, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97, KAT2A, GCN5, GCN5L2, HGCN5, PCAF-b, MGC102791.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
KAT2A (GCN5L2 or GCN5) is a histone acetyltransferase (HAT) that primarily acts as a transcriptional activator. GCN5L2 is potentially involved in acetylation in vivo. Expressed mainly during embryonic and newborn stages, GCN5 is vital for normal embryonic development, suggesting its role in differentiating embryonic-derived preadipocytes. GCN5 also represses NF-kappa-B by promoting ubiquitination of the NF-kappa-B subunit RELA in a HAT-independent manner. Loss of GCN5L2 leads to increased apoptosis in KAT2A mutants, starting before any visible morphological abnormalities.
Formulation
1mg/ml in PBS, pH 7.4, with 0.1% Sodium Azide.
Storage Procedures
Store at 4°C for up to 1 month. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
KAT2A antibody has been validated for specificity and reactivity through ELISA, Western blot, and Immunofluorescence analysis. However, optimal working dilutions should be determined empirically for each application. For Western blot and Immunofluorescence, a dilution range of 1:250 to 1:500 is recommended, with a starting dilution of 1:250.
Synonyms
Histone acetyltransferase KAT2A, General control of amino acid synthesis protein 5-like 2, Histone acetyltransferase GCN5, HsGCN5, Lysine acetyltransferase 2A, STAF97, KAT2A, GCN5, GCN5L2, HGCN5, PCAF-b, MGC102791.
Purification Method
KAT2A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT3G13AT.
Immunogen
Anti-human KAT2A mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human KAT2A amino acids 411-837 purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and κ light chain.
Product Science Overview
Mouse Anti-Human Antibodies
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human antigens. These antibodies are widely used in various research and diagnostic applications due to their specificity and versatility .
Applications
Mouse anti-human antibodies are commonly used in:
- ELISA (Enzyme-Linked Immunosorbent Assay): For detecting and quantifying specific antigens in a sample.
- Western Blotting: For identifying specific proteins in a complex mixture.
- Flow Cytometry: For analyzing the expression of cell surface and intracellular molecules.
- Immunohistochemistry: For visualizing the distribution and localization of specific proteins within tissue sections .
Advantages
These antibodies offer several advantages, including:
- High Specificity: They are affinity-purified to ensure high specificity for human immunoglobulins.
- Versatility: They can be used with various detection systems, such as HRP, AP, and fluorescence .
- Signal Amplification: Multiple secondary antibodies can bind to a single primary antibody, providing greater sensitivity through signal amplification .
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