KLK11 Human, Sf9
Sf9, Insect cells.
Kallikrein-11 isoform 1, KLK11, PRSS20, TLSP.
Greater than 90.0% as determined by SDS-PAGE.
Description
KLK11 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 241 amino acids (19-250a.a.) and having a molecular mass of 26.7 kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
KLK11 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Kallikreins are a subgroup of serine proteases with diverse physiological roles. Several kallikreins have been implicated in the development of cancer. Kallikrein-11 (KLK11) is a multifunctional protease and one of the 15 members of the kallikrein subfamily clustered on chromosome 19. KLK11 exhibits enzymatic activity by cleaving synthetic peptides after arginine residues, but not after lysine residues.
Produced in Sf9 insect cells, KLK11 is a single, glycosylated polypeptide chain comprising 241 amino acids (19-250a.a.). It has a molecular mass of 26.7 kDa; however, its apparent size on SDS-PAGE is estimated to be between 28-40 kDa. This KLK11 protein is expressed with a 9-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The KLK11 protein solution is provided at a concentration of 0.5 mg/mL in a buffer consisting of 50mM Tris-HCl (pH 7.5), 0.1M NaCl, 2mM CaCl2, and 10% glycerol.
The purity of KLK11 is determined to be greater than 90.0% by SDS-PAGE analysis.
Kallikrein-11 isoform 1, KLK11, PRSS20, TLSP.
Sf9, Insect cells.
ADLETRIIKG FECKPHSQPW QAALFEKTRL LCGATLIAPR WLLTAAHCLK PRYIVHLGQH NLQKEEGCEQ TRTATESFPH PGFNNSLPNK DHRNDIMLVK MASPVSITWA VRPLTLSSRC VTAGTSCLIS GWGSTSSPQL RLPHTLRCAN ITIIEHQKCE NAYPGNITDT MVCASVQEGG KDSCQGDSGG PLVCNQSLQG IISWGQDPCA ITRKPGVYTK VCKYVDWIQE TMKNNHHHHH H.
Product Science Overview
KLK11 is encoded by the KLK11 gene, which is located on chromosome 19. The protein is produced as a single, glycosylated polypeptide chain containing 241 amino acids (19-250a.a.) and has a molecular mass of approximately 26.7 kDa . The recombinant form of KLK11, produced in Sf9 insect cells, includes a 9 amino acid His tag at the C-terminus for purification purposes .
The recombinant KLK11 protein is expressed in Sf9 insect cells, a common system for producing recombinant proteins. The protein is purified using proprietary chromatographic techniques to achieve a purity greater than 90% as determined by SDS-PAGE . The protein is supplied as a sterile filtered, colorless solution containing 50mM Tris-HCl (pH 7.5), 0.1M NaCl, 2mM CaCl2, and 10% glycerol .
KLK11 is a multifunctional protease that cleaves synthetic peptides after arginine residues but not lysine residues . It is involved in various physiological processes, including the regulation of cell growth, tissue remodeling, and the inflammatory response. KLK11 has also been implicated in the progression of certain cancers, making it a potential biomarker for cancer diagnosis and prognosis .
For optimal stability, KLK11 should be stored at 4°C if used within 2-4 weeks. For longer storage periods, it is recommended to freeze the protein at -20°C and add a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation . Multiple freeze-thaw cycles should be avoided to maintain protein integrity.
