JSRP1 Human

Junctional Sarcoplasmic Reticulum Protein 1 Human Recombinant
Cat. No.
BT17515
Source
Escherichia Coli.
Synonyms
Junctional Sarcoplasmic Reticulum Protein 1, Homolog of Mouse Skeletal Muscle Sarcoplasmic Reticulum Protein JP-45, Junctional-Face Membrane Protein Of 45 KDa Homolog, JP45, 2310032K21Rik, Skeletal Muscle Sarcoplasmic Reticulum Protein JP-45.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

JSRP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 354 amino acids (1-331) and having a molecular mass of 38.0kDa.
JSRP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
JSRP1 is involved in the excitation-contraction coupling process within the sarcoplasmic reticulum. It interacts with CACNA1S, CACNB1, and calsequestrin to regulate calcium ion movement in skeletal muscle.
Description
Recombinant human JSRP1 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 354 amino acids (residues 1-331). It has a molecular weight of 38.0 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The JSRP1 solution is provided at a concentration of 0.25 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
Junctional Sarcoplasmic Reticulum Protein 1, Homolog of Mouse Skeletal Muscle Sarcoplasmic Reticulum Protein JP-45, Junctional-Face Membrane Protein Of 45 KDa Homolog, JP45, 2310032K21Rik, Skeletal Muscle Sarcoplasmic Reticulum Protein JP-45.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSMTTRA WEELDGGLGS CQALEDHSAL AETQEDRASA TPRLADSGSV PHDSQVAEGP SVDTRPKKME KEPAARGTPG TGKERLKAGA SPRSVPARKK AQTAPPLQPP PPPPALSEEL PWGDLSLNKC LVLASLVALL GSAFQLCRDA VPGEAALQAR VPEPWVPPSS APREPSSPLP KFEAQAPPSA PPAPRAEAEV RPKIPGSREA AENDEEEPGE ATGEAVREDR VTLADRGPKE RPRREGKPRK EKPRKEERPK KERPRKEERP RAAREPREAL PQRWESREGG HRPWARDSRD AEPRKKQAWV SPRRPDEEQR PGSRQKLRAG KGRD

Product Science Overview

Structure and Function

JSRP1 is involved in the regulation of the voltage-sensitive calcium channel CACNA1S. This regulation is essential for the proper influx and efflux of calcium ions in skeletal muscle cells, which is critical for muscle contraction and relaxation . The protein can interact with other key proteins such as CACNB1 and calsequestrin to help regulate calcium dynamics within the sarcoplasmic reticulum .

Recombinant Human JSRP1

Recombinant human JSRP1 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the human JSRP1 gene into a suitable host organism, such as Escherichia coli, to produce the protein in large quantities. The recombinant protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection .

The recombinant human JSRP1 protein is typically used in research to study its function and interactions with other proteins. It is also used in various assays to understand its role in muscle physiology and related diseases .

Clinical Significance

Mutations or dysregulation of the JSRP1 gene have been associated with certain muscular diseases. For example, atrophic muscular disease and Brody disease have been linked to abnormalities in JSRP1 . Understanding the function and regulation of JSRP1 is therefore important for developing potential therapeutic strategies for these conditions.

Research Applications

Recombinant human JSRP1 is used in various research applications, including:

  • Protein-Protein Interaction Studies: To investigate how JSRP1 interacts with other proteins involved in calcium regulation.
  • Functional Assays: To study the role of JSRP1 in muscle contraction and relaxation.
  • Disease Models: To understand how mutations in JSRP1 contribute to muscular diseases and to develop potential treatments .

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