JDP2 Human

Jun Dimerization Protein 2 Human Recombinant
Cat. No.
BT17202
Source
Escherichia Coli.
Synonyms
Jun dimerization protein 2, JDP2, JUNDM2, Progesterone Receptor Co-Activator.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

JDP2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids (1-163 a.a) and having a molecular mass of 21.2kDa.
JDP2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Jun dimerization protein 2 (JDP2) is a protein that is part of a larger group of proteins called the AP-1 transcription factor. This factor plays a role in controlling which genes are turned on or off in a cell. JDP2 specifically acts as a brake on the Jun family of proteins, preventing them from activating genes too strongly. JDP2 is involved in various cellular processes governed by AP-1, including cell death in response to UV damage, cell specialization, tumor development, and tumor suppression. It can also suppress gene activity by recruiting another protein called HDAC3 to specific regions of DNA. In essence, JDP2 regulates gene expression by directly influencing how DNA is packaged and modified.
Description
This product consists of the human JDP2 protein, manufactured in a lab using E. coli bacteria. The protein is not modified with sugar molecules (non-glycosylated) and is made up of a single chain of 187 amino acids (building blocks of proteins), with the first 163 being the actual JDP2 sequence. It has a molecular weight of 21.2kDa. For easier purification and handling, a 24 amino acid His-tag is attached to the beginning of the JDP2 protein. The purification process utilizes specialized chromatographic methods.
Physical Appearance
The product is a clear, colorless liquid that has been sterilized by filtration.
Formulation
The JDP2 protein solution has a concentration of 0.25mg/ml and is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 50% glycerol, 1mM DTT, 1mM EDTA, and 250mM imidazole.
Stability
For short-term storage (up to 2-4 weeks), keep the product refrigerated at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage to maintain protein stability. Avoid repeatedly freezing and thawing the product.
Purity
The purity of the JDP2 protein is greater than 90%, as determined by SDS-PAGE analysis, a technique used to separate and visualize proteins based on their size.
Synonyms
Jun dimerization protein 2, JDP2, JUNDM2, Progesterone Receptor Co-Activator.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMMPGQI PDPSVTTGSL PGLGPLTGLP SSALTVEELK YADIRNLGAM IAPLHFLEVK LGKRPQPVKS ELDEEEERRK RRREKNKVAA ARCRNKKKER TEFLQRESER LELMNAELKT QIEELKQERQ QLILMLNRHR PTCIVRTDSV KTPESEGNPL LEQLEKK.

Product Science Overview

Gene Location and Structure

The JDP2 gene is located on human chromosome 14q24.3. The gene spans approximately 46.4 kilobases, from base pair 75,427,715 to 75,474,111 . The protein itself consists of 163 amino acids and belongs to the basic leucine zipper (bZIP) family of transcription factors. The bZIP domain, which is essential for DNA binding and dimerization, includes amino acids from position 72 to 135 .

Function and Mechanism

JDP2 functions primarily as a transcriptional repressor. It dimerizes with c-Jun, another member of the AP-1 family, to repress AP-1-mediated transcriptional activation . This repression is crucial for regulating various cellular processes, including cell proliferation, differentiation, and apoptosis.

JDP2 also interacts with other transcription factors, such as Activating Transcription Factor 2 (ATF2), to modulate gene expression. It regulates the 12-O-tetradecanoylphorbol-13-acetate (TPA) response element (TRE) and cAMP-responsive element (CRE)-dependent transcription .

Biological Processes and Pathways

JDP2 is involved in several biological processes, including:

  • Regulation of Transcription: JDP2 negatively regulates transcription by RNA polymerase II and is involved in the positive regulation of histone deacetylation .
  • Cell Differentiation: It plays a role in the negative regulation of fat cell differentiation .
  • Response to Stress: JDP2 is part of the cellular response to oxidative stress and inflammation .
Clinical Significance

JDP2 has been implicated in various diseases and conditions. For instance, it is associated with granulomatous amebic encephalitis and primary amebic meningoencephalitis . Additionally, JDP2’s role in regulating oxidative stress and inflammation makes it a potential target for therapeutic interventions in cancer and other inflammatory diseases .

Recombinant JDP2

Recombinant JDP2 is produced using recombinant DNA technology, which involves inserting the JDP2 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. This recombinant protein is used in various research applications to study its function, interactions, and potential therapeutic uses.

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THE BIOTEK
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