JAZF1 Human

The JAZF1 gene encodes a protein that is involved in transcriptional repression. The protein consists of 266 amino acids and has a molecular mass of approximately 29.5 kDa . It is produced in Escherichia coli and is typically fused to a 23 amino acid His-tag at the N-terminus for purification purposes .

JAZF1 functions as a transcriptional corepressor for the orphan nuclear receptor NR2C2 . It inhibits the expression of the gluconeogenesis enzyme PCK2 by repressing NR2C2 activity . Additionally, JAZF1 is involved in the transcriptional activation of NAMPT by promoting the expression of PPARA and PPARD . This protein plays a significant role in lipid metabolism by suppressing lipogenesis, increasing lipolysis, and decreasing lipid accumulation in adipose tissue . It also contributes to glucose homeostasis by improving glucose metabolism and insulin sensitivity .

Chromosomal anomalies involving the JAZF1 gene are associated with endometrial stromal tumors . These anomalies can lead to the development of endometrial stromal sarcoma, a type of cancer that affects the connective tissue of the uterus . Additionally, JAZF1 has been linked to aneurysmal bone cysts .

The recombinant JAZF1 protein is produced in E. coli and is available as a non-glycosylated polypeptide chain . The protein solution typically contains 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA, and 10% glycerol . It is purified using proprietary chromatographic techniques to ensure a purity greater than 85% as determined by SDS-PAGE .

For optimal stability, the JAZF1 protein should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it is recommended to freeze the protein at -20°C with the addition of a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .