Encephalitis Japanese 12kda
Sterile Filtered clear solution.
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
Recombinant Japanese Encephalitis Virus produced in E. coli contains 110 amino acids and having a Mw of 12kDa. Encephalitis Japanese is fused to a His tag at its N-terminus and purified by proprietary chromatographic technique.
Product Specs
Product Science Overview
Japanese Encephalitis Virus (JEV) is a mosquito-borne flavivirus and a leading cause of viral encephalitis in Asia. The virus is primarily transmitted through the bite of infected Culex mosquitoes, which acquire the virus from pigs and water birds. JEV is responsible for significant morbidity and mortality, with approximately 50,000 cases and 15,000 deaths reported annually .
JEV has a single-stranded RNA genome of approximately 11 kb, encoding a single polyprotein that is processed into three structural proteins (C, prM, and E) and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) . The 12kDa protein in question is likely one of the non-structural proteins, specifically NS2B, which plays a crucial role in viral replication and assembly .
Recombinant proteins are produced by inserting the gene encoding the protein of interest into an expression system, such as bacteria, yeast, or mammalian cells. For JEV, the NS2B protein can be expressed in Escherichia coli (E. coli) with a hexahistidine tag at the N-terminus to facilitate purification . The recombinant NS2B protein can then be used for various biochemical and structural studies to understand its function and role in the viral life cycle.
The biochemical properties of recombinant JEV NS2B have been characterized to understand its role in the viral replication complex. NS2B is known to interact with the viral NS3 protein, forming a complex that exhibits protease activity essential for processing the viral polyprotein . This interaction is crucial for the maturation of the viral proteins and the assembly of new virions.
Studying recombinant JEV proteins, such as the 12kDa NS2B, is essential for developing antiviral strategies and vaccines. By understanding the structure-function relationship of these proteins, researchers can identify potential drug targets and design inhibitors to block viral replication. Additionally, recombinant proteins can be used in diagnostic assays to detect JEV infections and monitor immune responses in vaccinated individuals.
