JAK2 Antibody
Description
Product Specs
Product Science Overview
Janus Kinase 2 (JAK2) is a non-receptor tyrosine kinase that plays a crucial role in the signaling pathways of various cytokine receptors. It is a member of the Janus kinase family, which includes JAK1, JAK2, JAK3, and TYK2. JAK2 is involved in the signaling of type II cytokine receptors, the GM-CSF receptor family, the gp130 receptor family, and single-chain receptors .
JAK2 is characterized by the presence of seven Janus homology (JH) domains, labeled JH1 to JH7. The JH1 domain is the kinase domain responsible for its enzymatic activity, while the JH2 domain, initially thought to be a pseudokinase domain, has been found to have catalytic activity, albeit at a lower level . The JH2 domain plays a regulatory role, maintaining low basal activity in the absence of cytokines and facilitating activation upon cytokine binding .
JAK2 is activated upon cytokine binding to its receptor, leading to autophosphorylation and subsequent phosphorylation of signal transducer and activator of transcription (STAT) proteins. These phosphorylated STAT proteins then translocate to the nucleus, where they influence gene transcription . JAK2 is essential for the signaling of various cytokines and growth factors, including erythropoietin, thrombopoietin, and interleukins .
Mutations in the JAK2 gene have been implicated in several myeloproliferative disorders, including polycythemia vera, essential thrombocythemia, and myelofibrosis . The most clinically relevant mutation is V617F, which results in constitutive activation of JAK2 and hypersensitivity to cytokine signaling . This mutation is used as a diagnostic marker for polycythemia vera .
Mouse anti-human JAK2 antibodies are commonly used in research to study the expression and function of JAK2 in various biological contexts. These antibodies are generated by immunizing mice with human JAK2 protein, leading to the production of antibodies that specifically recognize and bind to human JAK2. These antibodies can be used in various applications, including Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the role of JAK2 in different signaling pathways and disease states .
