IYD Human

Iodotyrosine Deiodinase Human Recombinant

Cat. No.

BT25322

Source

Escherichia Coli.

Synonyms

Iodotyrosine dehalogenase 1, IYD-1, Iodotyrosine Deiodinase, IYD, C6orf71, DEHAL1, iodotyrosine dehalogenase 1 isoform 3, dJ422F24.1, TDH4.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

IYD Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 214 amino acids (24-214 a.a) and having a molecular mass of 25.1kDa.
IYD is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Iodotyrosine deiodinase (IYD) is an enzyme responsible for the removal of iodine from iodotyrosine molecules, specifically monoiodotyrosine (MIT) and diiodotyrosine (DIT). It exhibits a preference for MIT over DIT. This deiodination process, which requires NADPH as a cofactor, plays a crucial role in thyroid hormone synthesis. IYD, located on the membrane through its N-terminal anchor, facilitates iodide salvage during thyroglobulin breakdown. This salvaged iodide is then recycled for hormone production.

Description

This product consists of the recombinant human IYD enzyme, produced in E. coli. It is engineered as a single polypeptide chain, devoid of glycosylation. The protein encompasses amino acids 24 to 214 of the IYD sequence, with an additional 23 amino acid His-tag fused at the N-terminus, resulting in a molecular weight of 25.1 kDa. Purification is achieved using proprietary chromatographic methods.

Physical Appearance

The product is a clear, colorless solution that has been sterilized by filtration.

Formulation

The IYD protein is provided at a concentration of 1 mg/ml in a buffer solution containing 20mM Tris-HCl (pH 8.0), 0.4M urea, and 10% glycerol.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein such as 0.1% HSA or BSA is advisable for long-term storage. Repeated freezing and thawing should be avoided.

Purity

The purity of the IYD protein is greater than 90%, as determined by SDS-PAGE analysis.

Synonyms

Iodotyrosine dehalogenase 1, IYD-1, Iodotyrosine Deiodinase, IYD, C6orf71, DEHAL1, iodotyrosine dehalogenase 1 isoform 3, dJ422F24.1, TDH4.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSDRSMEKK KGEPRTRAEA RPWVDEDLKD SSDLHQAEED ADEWQESEEN VEHIPFSHNH YPEKEMVKRS QEFYELLNKR RSVRFISNEQ VPMEVIDNVI RTAGTAPSGA HTEPWTFVVV KDPDVKHKIR KIIEEEEEIN YMKRMGHRWV TDLKKLRTNW IKEYLDTAPI LILIFKQVHG FAANGKKKVH YYNE.

Product Science Overview

Introduction

Iodotyrosine deiodinase (IYD), also known as iodotyrosine dehalogenase 1, is a crucial enzyme involved in thyroid hormone metabolism. This enzyme is responsible for scavenging iodide by removing it from iodinated tyrosine residues in the thyroid gland. The iodide recovered by IYD is essential for the synthesis of thyroid hormones, which play a significant role in regulating metabolic rate, protein expression, and body temperature.

Structure and Function

IYD is a type of deiodinase enzyme that catalyzes the reductive dehalogenation of iodinated tyrosines, specifically monoiodotyrosine (MIT) and diiodotyrosine (DIT). These iodinated tyrosines are byproducts of thyroid hormone biosynthesis. The enzyme’s activity ensures that iodide is efficiently recycled within the thyroid gland, maintaining a balance of iodide and thyroid hormones.

Expression Patterns and Tissue Distribution

IYD is predominantly expressed in the thyroid gland, where it plays a vital role in thyroid hormone homeostasis. However, its expression is not limited to the thyroid; it is also found in other tissues such as the liver, kidney, and various parts of the gastrointestinal tract. This widespread distribution suggests that IYD may have additional roles beyond thyroid hormone metabolism.

Biological Functions

The primary function of IYD is to facilitate the recycling of iodide, which is crucial for the continuous production of thyroid hormones. Thyroid hormones, including thyroxine (T4) and triiodothyronine (T3), are essential for various physiological processes such as growth, development, and metabolism. By ensuring the availability of iodide, IYD helps maintain the proper functioning of the thyroid gland and the overall endocrine system.

Modes of Action

IYD operates through a mechanism known as reductive dehalogenation, which is distinct from the oxidative and hydrolytic dehalogenation processes observed in other aerobic organisms. This unique mode of action allows IYD to efficiently remove iodide from MIT and DIT, facilitating their conversion back into usable iodide for thyroid hormone synthesis.

Regulatory Mechanisms

The activity of IYD is tightly regulated to ensure optimal thyroid hormone levels. Various factors, including thyroid-stimulating hormone (TSH) and dietary iodide intake, influence the expression and activity of IYD. Additionally, feedback mechanisms involving thyroid hormones themselves help modulate IYD activity, maintaining a delicate balance within the thyroid gland.

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IYD Human

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