ITGB4 Human

Integrin Beta 4 Human Recombinant
Cat. No.
BT24827
Source
Sf9, Baculovirus cells.
Synonyms

Integrin beta-4, GP150, CD104, ITGB4, Integrin Subunit Beta 4, CD104 Antigen, Integrin, Beta 4, Integrin Beta-4.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

ITGB4 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 691 amino acids (28-710a.a.) and having a molecular mass of 77.5kDa. (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). ITGB4 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
ITGB4 (integrin beta-4 isoform 1) is a member of the Integrin beta family. It partners with Integrin alpha 6 to form noncovalent heterodimers, playing a crucial role in the formation of epithelial hemidesmosomes. These hemidesmosomes rely heavily on ITGB4 for structural integrity, and the protein is essential for regulating keratinocyte motility and polarity. Primarily associating with the alpha 6 subunit, ITGB4 is believed to have a significant impact on the behavior of invasive carcinoma.
Description
Produced in Sf9 Baculovirus cells, ITGB4 is a single, glycosylated polypeptide chain consisting of 691 amino acids (28-710a.a.). It has a molecular mass of 77.5kDa, although its size on SDS-PAGE may appear between 70-100kDa. The protein is expressed with an 8 amino acid His tag at the C-Terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ITGB4 protein solution is provided at a concentration of 0.5mg/ml and contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended periods, storage at -20°C in a frozen state is recommended. To ensure optimal long-term stability, adding a carrier protein like 0.1% HSA or BSA is advisable. Avoid subjecting the product to repeated freeze-thaw cycles.
Purity
The purity of ITGB4 is greater than 90.0% as determined by SDS-PAGE analysis.
Synonyms

Integrin beta-4, GP150, CD104, ITGB4, Integrin Subunit Beta 4, CD104 Antigen, Integrin, Beta 4, Integrin Beta-4.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence

NRCKKAPVKS CTECVRVDKD CAYCTDEMFR DRRCNTQAEL LAAGCQRESI VVMESSFQIT EETQIDTTLR RSQMSPQGLR VRLRPGEERH FELEVFEPLE SPVDLYILMD FSNSMSDDLD NLKKMGQNLA RVLSQLTSDY TIGFGKFVDK VSVPQTDMRP EKLKEPWPNS DPPFSFKNVI SLTEDVDEFR NKLQGERISG NLDAPEGGFD AILQTAVCTR DIGWRPDSTH LLVFSTESAF HYEADGANVL AGIMSRNDER CHLDTTGTYT QYRTQDYPSV PTLVRLLAKH NIIPIFAVTN YSYSYYEKLH TYFPVSSLGV LQEDSSNIVE LLEEAFNRIR SNLDIRALDS PRGLRTEVTS KMFQKTRTGS FHIRRGEVGI YQVQLRALEH VDGTHVCQLP EDQKGNIHLK PSFSDGLKMD AGIICDVCTC ELQKEVRSAR CSFNGDFVCG QCVCSEGWSG QTCNCSTGSL SDIQPCLREG EDKPCSGRGE CQCGHCVCYG EGRYEGQFCE YDNFQCPRTS GFLCNDRGRC SMGQCVCEPG WTGPSCDCPL SNATCIDSNG GICNGRGHCE CGRCHCHQQS LYTDTICEIN YSAIHPGLCE DLRSCVQCQA WGTGEKKGRT CEECNFKVKM VDELKRAEEV VVRCSFRDED DDCTYSYTME GDGAPGPNST VLVHKKKDCP PGSLEHHHHH H

Product Science Overview

Introduction

Integrin Beta 4 (ITGB4) is a protein encoded by the ITGB4 gene in humans. It is a subunit of integrin, a receptor that primarily binds to laminins, which are major components of the basement membrane . Integrin Beta 4 is known for its role in cell adhesion and signal transduction, particularly in epithelial cells.

Structure and Function

Integrin Beta 4 typically pairs with the Integrin Alpha 6 subunit to form the Integrin Alpha 6 Beta 4 complex . This complex plays a crucial role in the formation of hemidesmosomes, which are structures that anchor epithelial cells to the basement membrane . The integrin alpha-6/beta-4 complex is essential for maintaining the structural integrity of epithelial tissues and regulating keratinocyte polarity and motility .

Biological Significance

Integrin Beta 4 is involved in various biological processes, including:

  • Cell Adhesion: It helps in the adhesion of cells to the extracellular matrix, providing structural stability.
  • Signal Transduction: It participates in signaling pathways that regulate cell proliferation, differentiation, and survival.
  • Cancer Biology: Integrin Beta 4 is implicated in the progression of invasive carcinomas. Its overexpression is often associated with increased tumor invasiveness and poor prognosis .
Recombinant Integrin Beta 4

Recombinant Integrin Beta 4 is produced using recombinant DNA technology, which involves inserting the ITGB4 gene into a suitable expression system, such as Chinese Hamster Ovary (CHO) cells . This allows for the production of large quantities of the protein for research and therapeutic purposes.

Applications

Recombinant Integrin Beta 4 is used in various research applications, including:

  • Cancer Research: Studying the role of Integrin Beta 4 in tumor progression and metastasis.
  • Cell Biology: Investigating the mechanisms of cell adhesion and signal transduction.
  • Drug Development: Screening for potential therapeutic agents that target Integrin Beta 4.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.