ITGB3BP Human

Integrin Beta 3 Binding Protein Human Recombinant
Cat. No.
BT24731
Source
E.coli.
Synonyms
CENP-R, CENPR, HSU37139, NRIF3, TAP20, Centromere protein R, Beta-3-endonexin, Integrin beta-3-binding protein, Nuclear receptor-interacting factor 3, ITGB3BP.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ITGB3BP Human Recombinant produced in E. coli is a single polypeptide chain containing 239 amino acids (1-216) and having a molecular mass of 27.1kDa. ITGB3BP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Integrin Beta 3 Binding Protein (ITGB3BP1) is a protein found in many tissues. It has a DD1/RepD1 domain and an LXXLL motif. ITGB3BP1 acts as a coactivator for nuclear receptors when activated by estrogen. It is also associated with the CENP-A-CAD complex, which is involved in cell division, particularly the assembly of kinetochore proteins and chromosome segregation. Additionally, ITGB3BP1 can suppress gene transcription by interacting with the NFKB1 protein and hindering its ability to activate genes.
Description
Recombinant human ITGB3BP, produced in E. coli bacteria, is a single chain of 239 amino acids (specifically amino acids 1 to 216), with a molecular weight of 27.1 kDa. It has a 23 amino acid His-tag attached to its N-terminus and is purified using specialized chromatographic methods.
Physical Appearance
A clear, sterile solution.
Formulation
The ITGB3BP solution (0.5mg/ml) is supplied in a buffer containing 20mM Tris-HCl (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep at 4°C. For longer periods, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
More than 90% purity as determined by SDS-PAGE analysis.
Synonyms
CENP-R, CENPR, HSU37139, NRIF3, TAP20, Centromere protein R, Beta-3-endonexin, Integrin beta-3-binding protein, Nuclear receptor-interacting factor 3, ITGB3BP.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMPFAPVA QARVQWHDFR SLQHLLPAFK RFSCLSLGSS WDYSVKRSLK LDGLLEENSF DPSKITRKKS VITYSPTTGT CQMSLFASPT SSEEQKHRNG LSNEKRKKLN HPSLTESKES TTKDNDEFMM LLSKVEKLSE EIMEIMQNLS SIQALEGSRE LENLIGISCA SHFLKREMQK TKELMTKVNK QKLFEKSTGL PHKASRHLDS YEFLKAILN.

Product Science Overview

Introduction

Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. These proteins play a crucial role in cell adhesion and cell-surface-mediated signaling. Integrin Beta 3 (ITGB3) is one of the subunits that combine with various alpha subunits to form different integrins, each with distinct functions and properties .

Structure and Function

Integrin Beta 3 is commonly found in combination with the alpha IIb chain in platelets, forming the integrin αIIbβ3 complex. This complex is essential for platelet aggregation and blood clotting. Integrins, including αIIbβ3, are transmembrane glycoprotein receptors that transmit bioinformation bidirectionally across the plasma membrane. This bidirectional signaling is crucial for various cellular functions such as cell adhesion, spreading, migration, survival, differentiation, proliferation, and apoptosis .

Recombinant Human Integrin Beta 3

Recombinant human integrin beta 3 is produced using advanced biotechnological methods. Typically, it is expressed in Chinese Hamster Ovary (CHO) cells, which are widely used for the production of recombinant proteins due to their ability to perform complex post-translational modifications. The recombinant protein is purified to high levels of purity, often exceeding 90%, and is tested for its binding ability in functional assays .

Applications

Recombinant integrin beta 3 is used in various research applications, including:

  • Cell Adhesion Studies: Understanding the mechanisms of cell adhesion and migration.
  • Signal Transduction Research: Investigating the pathways involved in integrin-mediated signaling.
  • Drug Development: Screening potential therapeutic agents that target integrin beta 3 interactions.
  • Cancer Research: Studying the role of integrins in tumor cell proliferation and metastasis.
Therapeutic Targeting

Integrin αIIbβ3 is a promising target for antithrombotic therapy due to its central role in platelet functions and hemostasis. Therapeutic agents targeting this integrin can help prevent arterial thrombosis and other related conditions. The regulation of integrin αIIbβ3 involves numerous interacting proteins that associate with its cytoplasmic tails, making it a complex but valuable target for drug development .

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