ISOC2 Human

Isochorismatase Domain Containing 2 Human Recombinant
Cat. No.
BT16923
Source
Escherichia Coli.
Synonyms
Isochorismatase domain-containing protein 2, mitochondrial, ISOC2, Isochorismatase Domain Containing 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

ISOC2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (59-205) and having a molecular mass of 18kDa.
ISOC2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Isochorismatase Domain Containing 2, also known as ISOC2, belongs to the isochorismatase family. This protein is found in the mitochondrion, cytoplasm, and nucleus. ISOC2 interacts with CDKN2A and localizes to the nucleus in the presence of CDKN2A.
Description
Recombinant human ISOC2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 170 amino acids (59-205) with a molecular weight of 18 kDa. A 23 amino acid His-tag is fused to the N-terminus of ISOC2. Purification is carried out using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile filtered solution.
Formulation
The ISOC2 solution (0.5 mg/ml) is supplied in 20 mM Tris-HCl buffer (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
Isochorismatase domain-containing protein 2, mitochondrial, ISOC2, Isochorismatase Domain Containing 2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSEQYPQGL GPTVPELGTE GLRPLAKTCF SMVPALQQEL DSRPQLRSVL LCGIEAQACI LNTTLDLLDR GLQVHVVVDA CSSRSQVDRL VALARMRQSG AFLSTSEGLI LQLVGDAVHP QFKEIQKLIK EPAPDSGLLG LFQGQNSLLH.

Product Science Overview

Introduction

Isochorismatase Domain Containing 2 (ISOC2) is a protein-coding gene that encodes a protein known as Isochorismatase Domain-Containing Protein 2. This protein is involved in various cellular processes, including protein destabilization. The ISOC2 gene is located in the cytoplasm and nucleus of human cells .

Gene and Protein Information

The ISOC2 gene is a member of the isochorismatase family and has several aliases, including FLJ23469 and Isochorismatase Domain-Containing Protein 2, Mitochondrial . The gene is located on chromosome 19 and has been assigned the HGNC (HUGO Gene Nomenclature Committee) ID 26278 .

The protein encoded by the ISOC2 gene consists of 205 amino acids and has a molecular weight of approximately 23 kDa . It is expressed in various tissues and has been studied for its role in protein destabilization and other cellular functions .

Recombinant Protein

Recombinant human ISOC2 protein is produced using various expression systems, including wheat germ . This recombinant protein is used in research applications such as SDS-PAGE, ELISA, and Western Blotting (WB) . The recombinant protein is typically tag-free and is available in full-length form, making it suitable for various biochemical and biophysical studies .

Functional Analysis

The primary function of the ISOC2 protein is related to protein destabilization. This function is crucial for maintaining cellular homeostasis and regulating various cellular processes . The protein’s activity is influenced by its localization within the cell, primarily in the cytoplasm and nucleus .

Research Applications

Recombinant human ISOC2 protein is widely used in research to study its biochemical properties and functional roles. It is also used in assays to investigate protein-protein interactions, enzyme activity, and other cellular processes . The availability of high-quality recombinant protein allows researchers to conduct detailed studies on the protein’s structure and function.

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