IPP-POZ Human
(b) Analysis by SDS-PAGE.
Description
Product Specs
MANEDCPKAA DSPFSSDKHA QLILAQINKM RNGQHFCDVQ LQVGQESFKA HRLVLAASSPYFAALFTGGM KESSKDVVPI LGIEAGIFQI LLDFIYTGIV NIGVNNVQEL IIAADMLQLTEVVHLCCEFL KGQIDPLNCI GIFQFSEQIA CHDLLEF.
Product Science Overview
IPP is a 66 kDa protein composed of 584 amino acids . It contains two main domains:
- N-terminal POZ (BTB) Domain: The POZ (pox virus and zinc finger) domain, also known as the BTB (Broad-Complex, Tramtrack, and Bric à brac) domain, is located near the N-terminus of the protein. This domain is involved in protein-protein interactions and mediates homomeric and heteromeric dimerization . The POZ domain is also known to interact with components of histone deacetylase co-repressor complexes, including N-CoR and SMRT, and can mediate transcriptional repression .
- C-terminal Kelch Repeat Domain: This domain consists of six tandemly arranged repeats and is involved in various cellular functions, including actin binding .
The recombinant IPP-POZ protein is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 157 amino acids, with a molecular mass of approximately 17.3 kDa . The protein is purified using conventional chromatography techniques to achieve a purity greater than 95%, as determined by SDS-PAGE .
IPP-POZ (Human Recombinant) is primarily used in research settings. It is utilized in studies related to protein-protein interactions, transcriptional regulation, and cellular signaling pathways. The protein’s ability to interact with histone deacetylase co-repressor complexes makes it a valuable tool for investigating transcriptional repression mechanisms .
For short-term storage, the recombinant IPP-POZ protein should be kept at 4°C. For long-term storage, it is recommended to aliquot and store the protein at -20°C to avoid freeze-thaw cycles, which can degrade the protein . The protein is typically formulated in a buffer containing 10 mM HEPES (pH 7.4) and 25 mM NaCl .
