IP6K2 Human

Inositol Hexakisphosphate Kinase 2 Human Recombinant
Cat. No.
BT10675
Source
E.coli.
Synonyms
Inositol hexakisphosphate kinase 2 isoform a, IHPK2, PIUS, InsP6 kinase 2, P(i)-uptake stimulator, PiUS, IP6K2, TCCCIA00113.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IP6K2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 446 amino acids (1-426a.a) and having a molecular mass of 51.3kDa.
IP6K2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Inositol Hexakisphosphate Kinase 2 (IP6K2) is an enzyme within the inositol IPK family that catalyzes the conversion of inositol hexakisphosphate to diphosphoinositol pentakisphosphate. Additionally, IP6K2 facilitates the conversion of 1, 3, 4, 5, 6-pentakisphosphate (InsP5) to PP-InsP4. This protein-coding gene plays a role in suppressing tumor growth and influencing the apoptotic effects of IFN-beta in various ovarian cancers.
Description
Recombinant human IP6K2, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 446 amino acids (1-426a.a) with a molecular weight of 51.3 kDa. This protein is fused to a 20 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Colorless, sterile-filtered solution.
Formulation
The IP6K2 solution is provided at a concentration of 0.5 mg/ml in Tris-HCl buffer (pH 8.0) containing 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Inositol hexakisphosphate kinase 2 isoform a, IHPK2, PIUS, InsP6 kinase 2, P(i)-uptake stimulator, PiUS, IP6K2, TCCCIA00113.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSPAFRAMDV EPRAKGVLLE PFVHQVGGHS CVLRFNETTL CKPLVPREHQ FYETLPAEMR KFTPQYKGVV SVRFEEDEDR NLCLIAYPLK GDHGIVDIVD NSDCEPKSKL LRWTTNKKHH VLETEKTPKD WVRQHRKEEK MKSHKLEEEF EWLKKSEVLY YTVEKKGNIS SQLKHYNPWS MKCHQQQLQR MKENAKHRNQ YKFILLENLT SRYEVPCVLD LKMGTRQHGD DASEEKAANQ IRKCQQSTSA VIGVRVCGMQ VYQAGSGQLM FMNKYHGRKL SVQGFKEALF QFFHNGRYLR RELLGPVLKK LTELKAVLER QESYRFYSSS LLVIYDGKER PEVVLDSDAE DLEDLSEESA DESAGAYAYK PIGASSVDVR MIDFAHTTCR LYGEDTVVHE GQDAGYIFGL QSLIDIVTEI SEESGE.

Product Science Overview

Function and Mechanism

IP6K2 is primarily responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5) . This conversion is significant because InsP7 is involved in various cellular processes, including energy metabolism, apoptosis, and DNA repair . Additionally, IP6K2 can convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4 .

Biological Importance

The activity of IP6K2 is linked to several critical cellular functions:

  • Apoptosis: IP6K2 has been shown to affect the growth suppressive and apoptotic activities of interferon-beta in some ovarian cancers .
  • Energy Metabolism: By regulating the levels of inositol phosphates, IP6K2 plays a role in cellular energy homeostasis .
  • DNA Repair: InsP7, a product of IP6K2 activity, is involved in the DNA repair process, highlighting its importance in maintaining genomic stability .
Recombinant IP6K2

Recombinant human IP6K2 is produced using various expression systems, such as Escherichia coli . The recombinant protein is often tagged with a His tag at the N-terminus to facilitate purification and detection . The recombinant form retains the functional properties of the native protein, making it useful for research and therapeutic applications .

Applications in Research

Recombinant IP6K2 is widely used in biochemical and cellular studies to understand its role in inositol phosphate metabolism and its impact on cellular functions. It is also utilized in drug discovery and development, particularly in the context of cancer research, due to its involvement in apoptosis and growth suppression .

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