IF Human
Sterile Filtered pink solution.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Intrinsic Factor Human Recombinant produced in baculovirus is a glycosylated, polypeptide chain having a molecular mass of 55,000 Dalton.
The Intrinsic Factor is fused to a hexa-histidine at the C-terminus and purified by proprietary chromatographic techniques for removal of bound Vitamin B-12.
Product Specs
Recombinant Human Intrinsic Factor, a glycosylated polypeptide, is produced using baculovirus expression system. With a molecular weight of 55 kDa, it features a C-terminal hexa-histidine tag. Purification is achieved through proprietary chromatography techniques to eliminate bound Vitamin B-12.
Sterile, pink solution.
The protein is supplied in a solution containing 20mM HEPES buffer (pH 8.0), 100mM NaCl, and 20% Glycerol.
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC).
(b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Product Science Overview
Vitamin B12 is essential for various bodily functions, including DNA synthesis and red blood cell formation. However, it cannot be absorbed directly by the body. When vitamin B12 enters the stomach, it binds to a protein called haptocorrin. This complex travels to the duodenum, where pancreatic enzymes digest haptocorrin, freeing vitamin B12. In the less acidic environment of the small intestine, vitamin B12 binds to intrinsic factor. This new complex then travels to the ileum, where specialized epithelial cells endocytose it. Inside the cell, vitamin B12 dissociates from intrinsic factor and binds to another protein, transcobalamin II, which transports it to the liver .
Recombinant Human Intrinsic Factor is a laboratory-produced version of the naturally occurring protein. It is created using recombinant DNA technology, which involves inserting the gene responsible for producing intrinsic factor into a host cell, such as HEK293 cells. These cells then produce the protein, which can be harvested and purified for various applications .
Recombinant Human Intrinsic Factor is used in research and clinical diagnostics. It serves as a high-quality replacement for native porcine intrinsic factor, which has seen a decline in quality in recent years. The recombinant version is highly purified and offers a reliable and economical alternative. It is particularly useful in vitamin B12 assay development and other research applications .
