IFN tau Ovine
IFN-tau1, Trophoblast protein 1, TP-1, Trophoblastin, Antiluteolysin, Trophoblast antiluteolytic protein, IFN-tau, IFN tau-1.
Sterile Filtered White lyophilized (freeze-dried) powder.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
IFN-Tau Ovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 19914.7 Dalton.
The IFN-Tau is purified by proprietary chromatographic techniques.
Product Specs
IFN-tau1, Trophoblast protein 1, TP-1, Trophoblastin, Antiluteolysin, Trophoblast antiluteolytic protein, IFN-tau, IFN tau-1.
Product Science Overview
IFN-τ was discovered as a unique interferon due to its role in pregnancy recognition in sheep. Unlike other type I interferons, IFN-τ has potent immunosuppressive and antiviral activities but is less cytotoxic . This makes it a promising candidate for therapeutic applications, particularly in modulating immune responses and treating viral infections.
The recombinant form of ovine IFN-τ (rOvIFN-τ) is produced using genetic engineering techniques. The gene encoding IFN-τ is cloned and expressed in a suitable host, such as Escherichia coli (E. coli), to produce large quantities of the protein. The process involves several steps :
- Gene Cloning: The IFN-τ gene is extracted from sheep embryonic tissues and amplified using reverse transcription-polymerase chain reaction (RT-PCR).
- Vector Construction: The amplified gene is inserted into a plasmid vector, such as pET-32a (+), which is then introduced into E. coli cells.
- Protein Expression: The E. coli cells are induced to express the recombinant protein using isopropyl-beta-D-thiogalactopyranoside (IPTG).
- Protein Purification: The expressed protein is purified using techniques like nickel affinity chromatography and DEAE anion exchange chromatography to obtain a high-purity product.
Recombinant ovine IFN-τ exhibits several biological activities, including :
- Immunomodulation: It modulates the expression of major histocompatibility complex (MHC) class I and II molecules, which are crucial for immune responses.
- Antiviral Activity: It inhibits the replication of viruses, such as vesicular stomatitis virus (VSV) and Theiler’s virus, in various cell types.
- Anti-inflammatory Effects: It downregulates the expression of MHC class II molecules induced by interferon-gamma (IFN-γ), which may help in reducing inflammation in the central nervous system (CNS).
Due to its unique properties, recombinant ovine IFN-τ has potential therapeutic applications in various fields :
- Pregnancy Support: It can be used to support pregnancy in livestock by enhancing maternal recognition of pregnancy.
- Immune Modulation: Its immunosuppressive properties make it a candidate for treating autoimmune diseases and reducing inflammation.
- Antiviral Therapy: Its antiviral activity can be harnessed to develop treatments for viral infections.
In conclusion, IFN-τ is a unique and versatile interferon with significant potential for therapeutic applications. Its recombinant production allows for large-scale availability, making it a valuable tool for research and clinical use.
