Intein Bacillus Circulans
Description
Intein is fused to a 16 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Intein Bacillus Circulans Recombinant is a protein derived from the bacterium Bacillus circulans. Inteins are segments of proteins that can excise themselves from a larger protein sequence and join the remaining portions with a peptide bond. This self-excision and ligation process is known as protein splicing. Inteins also contain an endonuclease domain, which plays a role in intein proliferation by facilitating the insertion of intein-coding sequences into new genomic locations.
Bacillus circulans is a gram-positive, rod-shaped bacterium belonging to the phylum Firmicutes. It can exist as an obligate aerobe or a facultative anaerobe, meaning it can survive in both oxygen-rich and oxygen-poor environments. Bacillus circulans can produce oval endospores, allowing it to remain dormant under stressful conditions for extended periods. This bacterium is known for producing large amounts of enzymes, making it valuable in various industrial applications .
The recombinant form of Intein Bacillus Circulans is produced in Escherichia coli. The recombinant protein is a single, non-glycosylated polypeptide chain containing 533 amino acids and has a molecular mass of 59.4 kilodaltons. The intein is fused to a 16 amino acid histidine tag at the C-terminus, which facilitates purification using chromatographic techniques .
The recombinant Intein Bacillus Circulans is formulated in a solution containing 20 millimolar Tris-HCl buffer (pH 8.0), 1 millimolar dithiothreitol, 0.1 molar sodium chloride, and 10% glycerol. The protein solution is sterile-filtered and colorless. For short-term storage, it can be kept at 4°C, while for long-term storage, it should be frozen at -20°C. To prevent degradation during long-term storage, it is recommended to add a carrier protein such as human serum albumin or bovine serum albumin .
Recombinant Intein Bacillus Circulans has various applications in research and industry. It is used in protein purification, where the intein-mediated cleavage allows for the removal of fusion tags without the need for proteases. This protein is also utilized in studying protein splicing mechanisms and intein biology. Additionally, it has potential applications in biotechnology, such as the development of novel protein-based therapeutics and bioengineering .
