Insulin Antibody
Description
Product Specs
Purified by chromatography on protein A chromatography.
IgG1 kappa.
Product Science Overview
Insulin is a crucial hormone in the regulation of glucose metabolism, produced by the beta cells of the pancreas. It plays a vital role in maintaining blood glucose levels by facilitating the uptake of glucose into cells for energy production and storage. The study of insulin and its interactions is essential for understanding and treating diabetes and other metabolic disorders.
Insulin is a peptide hormone composed of 51 amino acids, arranged in two chains (A and B) linked by disulfide bonds. The A chain consists of 21 amino acids, while the B chain has 30 amino acids. Insulin is initially synthesized as a single polypeptide called preproinsulin, which undergoes several processing steps to become mature insulin.
Mouse anti-human insulin antibodies are monoclonal antibodies produced by immunizing mice with human insulin. These antibodies are highly specific to human insulin and are used in various research and diagnostic applications. They are particularly valuable in immunohistochemistry (IHC), enzyme-linked immunosorbent assays (ELISA), Western blotting, and flow cytometry.
- Immunohistochemistry (IHC): These antibodies are used to detect and visualize insulin in tissue sections. This application is crucial for studying the distribution and localization of insulin-producing cells in the pancreas and other tissues.
- Enzyme-Linked Immunosorbent Assay (ELISA): ELISA is a widely used technique for quantifying insulin levels in biological samples. Mouse anti-human insulin antibodies are used as capture or detection antibodies in these assays.
- Western Blotting: In Western blotting, these antibodies help detect insulin in protein extracts from cells or tissues. This technique is essential for studying insulin expression and its regulation under various conditions.
- Flow Cytometry: Flow cytometry allows for the analysis of insulin-producing cells at the single-cell level. Mouse anti-human insulin antibodies are used to label and quantify these cells in mixed cell populations.
- High Specificity: These antibodies are highly specific to human insulin, ensuring accurate detection and quantification.
- Versatility: They can be used in various applications, including IHC, ELISA, Western blotting, and flow cytometry.
- Reproducibility: Monoclonal antibodies provide consistent and reproducible results, making them reliable tools for research and diagnostics.
