INHBC Human

Inhibin-Beta C Chain Human Recombinant
Cat. No.
BT14843
Source
E.coli.
Synonyms

Inhibin Beta C, Actv Beta-C Chain, IHBC, Inhibin Beta C Chain.

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

INHBC Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 139 amino acids (237-352) and having a molecular mass of 14.9kDa.
INHBC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
INHBC, also known as inhibin beta C chain, is a member of the transforming growth factor beta (TGF-β) superfamily. It combines with beta A and beta B subunits to form heterodimers. The TGF-β superfamily includes activins and inhibins, hormones with opposing roles in regulating hormone secretion from the pituitary, hypothalamus, and gonads. They are also involved in the differentiation and growth of various cell types.
Description
Recombinant human INHBC, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 139 amino acids (residues 237-352), resulting in a molecular weight of 14.9 kDa. For purification purposes, it is tagged with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The INHBC solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 85% using SDS-PAGE analysis.
Synonyms

Inhibin Beta C, Actv Beta-C Chain, IHBC, Inhibin Beta C Chain.

Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSGIDCQGG SRMCCRQEFF VDFREIGWHD WIIQPEGYAM NFCIGQCPLH IAGMPGIAAS FHTAVLNLLK ANTAAGTTGG GSCCVPTARR PLSLLYYDRD SNIVKTDIPD MVVEACGCS 

Product Science Overview

Introduction

Inhibin-Beta C Chain, also known as INHBC, is a protein encoded by the INHBC gene in humans. This protein is a member of the transforming growth factor-beta (TGF-beta) superfamily, which plays a crucial role in various cellular processes, including growth, differentiation, and hormone regulation .

Gene and Protein Structure

The INHBC gene is located on chromosome 12 (12q13.3) in humans . The gene encodes the beta C chain of inhibin, which can form heterodimers with other inhibin subunits, such as beta A and beta B . The human recombinant form of Inhibin-Beta C Chain is produced in E. coli and consists of a single, non-glycosylated polypeptide chain containing 139 amino acids, with a molecular mass of approximately 14.9 kDa .

Function and Mechanism

Inhibins and activins, both members of the TGF-beta superfamily, have opposing actions. Inhibins inhibit the secretion of follicle-stimulating hormone (FSH) by the pituitary gland, while activins stimulate its secretion . The Inhibin-Beta C Chain forms homodimers and heterodimers, which are involved in regulating various functions such as hormone secretion, germ cell development, erythroid differentiation, insulin secretion, nerve cell survival, and bone growth .

Expression and Clinical Significance

The expression of Inhibin-Beta C Chain is predominant in adult liver and in conditions such as benign prostatic hyperplasia . Transgenic mice overexpressing the INHBC gene exhibit defects in the testis, liver, and prostate, indicating its significant role in these organs . Additionally, the INHBC gene is involved in pathways related to peptide hormone metabolism and protein metabolism .

Industrial Production

The recombinant form of Inhibin-Beta C Chain is produced using proprietary chromatographic techniques. It is fused to a 23 amino acid His-tag at the N-terminus to facilitate purification . This recombinant protein is used in various research applications to study its role in cellular processes and its potential therapeutic applications.

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