IMPDH2 Human

IMP Dehydrogenase 2 Human Recombinant
Cat. No.
BT10534
Source
E.coli.
Synonyms
Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, IMPDH-II, IMPDH2, IMPD2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IMPDH2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 534 amino acids (1-514) and having a molecular mass of 58kDa.
IMPDH2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
IMPDH2, a member of the IMPDH/GMPR family, catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate to xanthine-5'-monophosphate, which is then converted to guanosine-5'-monophosphate. As the rate-limiting enzyme in de novo guanine nucleotide biosynthesis, IMPDH2 is crucial for maintaining cellular guanine deoxy- and ribonucleotide pools necessary for DNA and RNA synthesis. Both IMPDH1 and IMPDH2 are targets of the immunosuppressive drug, MPA. Notably, IMPDH2 gene up-regulation is observed in some neoplasms, suggesting a potential role in malignant transformation.
Description
Recombinant Human IMPDH2, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 534 amino acids (residues 1-514). With a molecular mass of 58 kDa, it features a 20 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution, sterile-filtered.
Formulation
The IMPDH2 solution (0.5 mg/ml) is formulated in 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 20% glycerol, and 150 mM NaCl.
Stability
For short-term storage (up to 4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 90% as determined by SDS-PAGE analysis.
Synonyms
Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, IMPD 2, IMPDH 2, IMPDH-II, IMPDH2, IMPD2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MADYLISGGT SYVPDDGLTA QQLFNCGDGL TYNDFLILPG YIDFTADQVD LTSALTKKIT LKTPLVSSPM DTVTEAGMAI AMALTGGIGF IHHNCTPEFQ ANEVRKVKKY EQGFITDPVV LSPKDRVRDV FEAKARHGFC GIPITDTGRM GSRLVGIISS RDIDFLKEEE HDCFLEEIMT KREDLVVAPA GITLKEANEI LQRSKKGKLP IVNEDDELVA IIARTDLKKN RDYPLASKDA KKQLLCGAAI GTHEDDKYRL DLLAQAGVDV VVLDSSQGNS IFQINMIKYI KDKYPNLQVI GGNVVTAAQA KNLIDAGVDA LRVGMGSGSI CITQEVLACG RPQATAVYKV SEYARRFGVP VIADGGIQNV GHIAKALALG ASTVMMGSLL AATTEAPGEY FFSDGIRLKK YRGMGSLDAM DKHLSSQNRY FSEADKIKVA QGVSGAVQDK GSIHKFVPYL IAGIQHSCQD IGAKSLTQVR AMMYSGELKF EKRTSSAQVE GGVHSLHSYE KRLF.

Product Science Overview

Structure and Isoforms

IMPDH2 belongs to the IMPDH/GMPR family and is one of two isoforms of IMP dehydrogenase found in humans . The other isoform, IMPDH1, is generally constitutively expressed, whereas IMPDH2 is inducible during cellular proliferation and transformation . The two isoforms share 84% sequence identity but are distinctly regulated .

Function and Importance

IMPDH2 plays a pivotal role in maintaining the cellular pools of guanine nucleotides, which are essential for DNA and RNA synthesis . The enzyme’s activity is crucial for rapidly proliferating cells, such as lymphocytes and tumor cells . Due to its central role in nucleotide biosynthesis, IMPDH2 is a validated target for several therapeutic applications, including antiviral, antiparasitic, antimicrobial, antileukemic, and immunosuppressive treatments .

Recombinant Human IMPDH2

Recombinant human IMPDH2 is produced using various expression systems, including E. coli and baculovirus-infected insect cells . The recombinant enzyme retains its catalytic activity and is used in research to study its function and to screen for potential inhibitors . The enzyme’s activity is measured by its ability to convert IMP to XMP, with specific activity values provided under defined assay conditions .

Therapeutic Applications

IMPDH2 inhibitors, such as mycophenolic acid (MPA) and ribavirin, are used to treat a wide range of diseases . MPA, for example, is an immunosuppressive agent used to prevent organ transplant rejection and to treat autoimmune diseases . The inhibition of IMPDH2 leads to a decrease in guanine nucleotide levels, which in turn disrupts nucleic acid synthesis in proliferating cells .

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