IMP3 Human

IMP3 Human Recombinant
Cat. No.
BT16151
Source
E.coli.
Synonyms
BRMS2, C15orf12, MRPS4, U3 snoRNP protein IMP3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

IMP3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids (1-184 a.a) and having a molecular mass of 24kDa. IMP3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
IMP3, the human counterpart of the yeast Imp3 protein, is crucial for early cleavages in pre-18S ribosomal RNA processing. This protein is a component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP) and belongs to the ribosomal protein S4P family. IMP3 is found in the nucleoli, interacts with the U3 snoRNP complex, and possesses an S4 domain. The IMP3 gene encodes this U3 small nucleolar ribonucleoprotein.
Description
Recombinant human IMP3, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 207 amino acids (residues 1-184) with a molecular weight of 24kDa. The protein is expressed with a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
IMP3 protein solution at a concentration of 1mg/ml in 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Synonyms
BRMS2, C15orf12, MRPS4, U3 snoRNP protein IMP3.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMVRKLKF HEQKLLKQVD FLNWEVTDHN LHELRVLRRY RLQRREDYTR YNQLSRAVRE LARRLRDLPE RDQFRVRASA ALLDKLYALG LVPTRGSLEL CDFVTASSFC RRRLPTVLLK LRMAQHLQAA VAFVEQGHVR VGPDVVTDPA FLVTRSMEDF VTWVDSSKIK RHVLEYNEER DDFDLEA.

Product Science Overview

Structure and Function

IMP3 contains two RNA recognition motifs and four K homology domains, which are crucial for its function in binding to RNA. These structural features enable IMP3 to interact with specific mRNA targets, influencing their localization within the cell, their stability, and their translation into proteins .

Biological Role

IMP3 is an oncofetal protein, meaning it is typically expressed during fetal development and in various types of cancer. It plays a significant role in cell growth and development, and its expression is often associated with poor prognosis and metastasis in several cancers . Research has shown that IMP3 is involved in a variety of physiological and pathological processes, including:

  • Growth and Development: IMP3 is essential for normal cellular growth and development. It regulates the expression of genes involved in these processes by binding to their mRNAs .
  • Cancer: IMP3 is highly expressed in many tumor types, including testicular and melanocytic neoplasia. Its expression is often used as a marker for poor prognosis in cancer patients .
  • Stem Cells: In human pluripotent stem cells (hPSCs), IMP3 is involved in maintaining the precise control of post-transcriptional RNA networks, which are crucial for the proliferation and survival of these cells .
Clinical Significance

The expression of IMP3 has been studied extensively in the context of cancer. It is considered a marker for poor prognosis and metastasis in various cancers, including testicular and melanocytic neoplasia . Additionally, IMP3 has been proposed as a diagnostic marker for differentiating certain types of lymphomas, such as nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) from T cell/histiocyte-rich large B cell lymphoma (THRLBCL) .

Research and Applications

IMP3 is widely used in research to study its role in cancer and development. Recombinant IMP3, produced through recombinant DNA technology, is used in various experimental applications, including:

  • Western Blotting: To detect the presence and levels of IMP3 in different cell lines and tissues .
  • Immunohistochemistry: To study the expression patterns of IMP3 in tissue samples, particularly in cancer research .
  • RNA-binding Studies: To identify and characterize the RNA targets of IMP3 in different cellular contexts .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

IMP3 Antibody
IMP3, Mouse Anti Human
Cat. No.
BT21129
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.