IL 11 Mouse

Interleukin-11 Mouse Recombinant
Cat. No.
BT30425
Source
Escherichia Coli.
Synonyms

AGIF, Adipogenesis inhibitory factor, IL-11, Interleukin-11, Il11.

Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Interleukin-11 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 19.1kDa.
The Mouse IL-11 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Interleukin 11 (IL-11) is a cytokine belonging to the gp130 family, known for their role in assembling multi-subunit receptor complexes. These complexes always include at least one molecule of the transmembrane signaling receptor IL6ST (gp130). IL-11 has been shown to promote the development of antibody-producing B cells in a T-cell-dependent manner. Furthermore, it supports the growth and proliferation of hematopoietic stem cells and megakaryocyte progenitor cells.
Description
Recombinant Mouse Interleukin-11, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 179 amino acids. It has a molecular weight of 19.1 kDa. The purification of Mouse IL-11 is achieved through proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The protein was freeze-dried from a 0.2 µm filtered solution at a concentration of 1 mg/ml in phosphate-buffered saline (PBS) at pH 7.4.
Solubility
To reconstitute the lyophilized Interleukin 11, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized Interleukin-11 remains stable at room temperature for up to 3 weeks; however, it is recommended to store it desiccated at a temperature below -18°C. After reconstitution, IL11 should be stored at 4°C for 2 to 7 days. For long-term storage, it is advisable to store it at -18°C. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is recommended. It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of this product is greater than 97.0%, as determined by the following methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity of this product is measured by its ability to stimulate the proliferation of murine T11 cells in a dose-dependent manner. The ED₅₀, defined as the concentration required to achieve half-maximal proliferation, was determined to be less than 2.0 ng/ml. This corresponds to a specific activity of 500,000 IU/mg.
Synonyms

AGIF, Adipogenesis inhibitory factor, IL-11, Interleukin-11, Il11.

Source
Escherichia Coli.
Amino Acid Sequence
MPGPPAGSPR VSSDPRADLD SAVLLTRSLL ADTRQLAAQM RDKFPADGDH SLDSLPTLAM SAGTLGSLQL PGVLTRLRVD LMSYLRHVQW LRRAGGPSLK TLEPELGALQ ARLERLLRRL QLLMSRLALP QAAPDQPVIP LGPPASAWGS IRAAHAILGG LHLTLDWAVR GLLLLKTRL.

Product Science Overview

Biological Functions

IL-11 is known for its anti-apoptotic, anti-inflammatory, and hematopoietic potential . It plays a crucial role in the differentiation of hematopoietic cells, including progenitor cells, and is involved in megakaryocytopoiesis, thrombopoiesis, erythropoiesis, and myelopoiesis . Additionally, IL-11 has been shown to have effects on various cell types in the liver, gastrointestinal tract, lung, heart, central nervous system, bone, joint, and immune system .

Receptor and Signaling

The activity of IL-11 is determined by the expression of the IL-11 receptor alpha (IL-11Rα) and the signal transducing subunit beta (gp130) on the cell membrane . When IL-11 binds to its receptor, it forms a complex with gp130, which then initiates intracellular signaling pathways that lead to its various biological effects .

Recombinant IL-11

Recombinant mouse IL-11 is produced using various expression systems, including E. coli . The recombinant protein is often supplied in a lyophilized form and is used in research to study its biological effects and potential therapeutic applications .

Therapeutic Potential

IL-11 has been explored for its potential therapeutic applications in various conditions. It has shown promise in the treatment of thrombocytopenia, infertility, multiple sclerosis, cardiovascular diseases, and inflammatory disorders . Additionally, IL-11 has displayed anti-melanoma activity when used as a molecular adjuvant in therapeutic whole-cell melanoma vaccine formulations .

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