IL 29 Human, Sf9

Interleukin-29 (IL-29), also known as IFN-Lambda 1, is a member of the type III interferon family. It is distantly related to type I interferons and the IL-10 family. IL-29 is produced in response to viral infections and plays a crucial role in the innate immune response by activating monocytes and macrophages .

Human recombinant IL-29 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 187 amino acids, with a molecular mass of approximately 20.8 kDa . The recombinant protein is fused with a 6-amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .

IL-29 exhibits several biological activities similar to type I interferons, including antiviral, antiproliferative, and antitumor activities . However, its effectiveness is generally lower and limited to a narrower range of cell lines compared to type I interferons . IL-29 interacts with a heterodimeric class II cytokine receptor composed of interleukin 10 receptor, beta (IL10RB), and interleukin 28 receptor, alpha .

IL-29 induces the expression of ELR(-) CXC chemokine mRNA in human peripheral blood mononuclear cells in an IFN-gamma-independent manner . It is capable of generating tolerogenic dendritic cells (DCs), which can counteract the functions of IFN-beta . This cytokine is essential for activating innate immune responses at the site of viral infection by producing a restricted panel of cytokines .

Recombinant IL-29 is used extensively in laboratory research to study its role in immune responses and its potential therapeutic applications. It is particularly valuable in understanding the mechanisms of antiviral defense and the development of novel antiviral therapies .

IL-29 protein solution should be stored at 4°C if used within 2-4 weeks. For longer storage, it should be frozen at -20°C, with the addition of a carrier protein (0.1% HSA or BSA) to prevent multiple freeze-thaw cycles . The protein is stable for long-term storage under these conditions .