IL 24 Human
Interleukin-24 Human Recombinant
Cat. No.
BT2982
Source
Sacharomyces cerevisiae.
Synonyms
C49A, FISP, MDA7, ST16, IL-24, IL10B, Mob-5, MDA-7, Suppression of tumorigenicity 16 protein, Melanoma differentiation-associated gene 7 protein.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Interleukin 24 human recombinant produced in yeast is a single, glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 18 kDa. As a result of glycosylation, the protein migrates at 19.5 kDa on SDS-PAGE.
Product Specs
Introduction
Interleukin 24 (IL-24), a cytokine belonging to the IL-10 family, was initially discovered as a gene upregulated during the terminal differentiation of melanoma cells. IL-24 exhibits selective apoptosis induction in various cancer cells. Its overexpression elevates the expression of multiple GADD family genes, correlating with apoptosis induction. IL-24 also triggers the phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38) and heat shock 27kDa protein 1 (HSPB2/HSP27) in melanoma cells, a phenomenon not observed in normal immortal melanocytes. Notably, alternative splicing of IL-24 transcripts generates isoforms with distinct properties. Glycosylation is crucial for IL-24's functional activity. Functionally, IL-24 displays diverse activities. At lower concentrations, it promotes the production of type I proinflammatory cytokines, including IFN-g, IL-1b, IL-12, and TNF-a. Conversely, at higher concentrations, IL-24 acts as a potent inducer of apoptosis specifically in tumor cells while sparing normal cells. These remarkable properties have earned mda-7/IL-24 recognition as a potential 'magic bullet' for cancer gene therapy.
Description
Recombinant human Interleukin 24, produced in yeast, is a single-chain glycosylated polypeptide comprising 158 amino acids. This protein exhibits a molecular mass of 18 kDa. Due to glycosylation, it migrates at 19.5 kDa on SDS-PAGE.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
Lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) as a carrier.
Solubility
To reconstitute the lyophilized IL-24, it is recommended to dissolve it in sterile 18 MΩ-cm H2O at a concentration of at least 100 µg/ml. This solution can then be further diluted in other aqueous solutions.
Stability
Lyophilized MDA7, while stable at room temperature for up to 3 weeks, should be stored desiccated at temperatures below -18°C. Upon reconstitution, recombinant MDA-7 should be stored at 4°C for 2-7 days. For long-term storage, it is recommended to store it below -18°C. To ensure optimal stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 98.0% using the following methods:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC) analysis.
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
Biological Activity
The biological activity is assessed based on the protein's ability to bind to the cell receptor of the Capan-1 cell line, resulting in Stat-3 activation. Typically, the effective concentration for 50% activation (ED50) is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.
Synonyms
C49A, FISP, MDA7, ST16, IL-24, IL10B, Mob-5, MDA-7, Suppression of tumorigenicity 16 protein, Melanoma differentiation-associated gene 7 protein.
Source
Sacharomyces cerevisiae.
Product Science Overview
Structure and Production
IL-24 is produced by various cell types, including myeloid and lymphoid cells, as well as tumor cells . The recombinant form of IL-24 is typically produced in cell cultures, such as CHO (Chinese Hamster Ovary) cells or mouse myeloma cell lines . The recombinant protein is often optimized for use in cell culture, differentiation studies, and functional assays .
Receptors and Signaling
IL-24 exerts its effects by binding to specific heterodimeric receptors, namely IL-20R1/IL-20R2 and IL-22R1/IL-20R2 . Upon binding to these receptors, IL-24 activates various signaling pathways, including the JAK1/STAT3 phosphorylation cascade . This signaling is crucial for controlling cell differentiation, apoptosis, and proliferation.
Biological Functions
IL-24 has several important biological functions:
- Tumor Suppression: IL-24 is known for its ability to induce apoptosis selectively in cancer cells without affecting healthy cells . This makes it a promising candidate for cancer therapy.
- Immune Modulation: IL-24 plays a role in immune surveillance by acting on immune cells and non-hematopoietic tissues . It helps in modulating the immune response against tumors.
- Angiogenesis and Wound Healing: IL-24 is involved in the regulation of angiogenesis (formation of new blood vessels) and wound healing .
Applications
Recombinant IL-24 is used in various research applications, including:
- In Vitro Studies: It is used to study tumor cell immunosuppression, angiogenesis, and cell proliferation .
- Cytokine-Receptor Signaling: Researchers use IL-24 to investigate cytokine-receptor signaling pathways and their effects on cell behavior .
- Cancer Research: Due to its tumor-suppressive properties, IL-24 is extensively studied for its potential in cancer therapy .
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