IGJ Human

Immunoglobulin J Human Recombinant
Cat. No.
BT23141
Source
E.coli.
Synonyms
IGCJ, JCH, Immunoglobulin J chain, IGJ.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IGJ Human Recombinant produced in E. coli is a single polypeptide chain containing 160 amino acids (23-159) and having a molecular mass of 18kDa. IGJ is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Immunoglobulin J chain (IGJ) plays a crucial role in linking two monomer units of IgM or IgA antibodies. It facilitates the binding of these immunoglobulins to the secretory component. In the context of IgM, the J chain-joined dimer serves as the foundation for the pentameric structure, while in IgA, it promotes the formation of larger polymers.
Description
Recombinant human IGJ, produced in E. coli, is a single polypeptide chain consisting of 160 amino acids (residues 23-159) with a molecular weight of 18 kDa. The protein includes a 23-amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The IGJ solution is provided at a concentration of 0.5 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 10% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 85% as determined by SDS-PAGE analysis.
Synonyms
IGCJ, JCH, Immunoglobulin J chain, IGJ.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSQEDERIV LVDNKCKCAR ITSRIIRSSE DPNEDIVERN IRIIVPLNNR ENISDPTSPL RTRFVYHLSD LCKKCDPTEV ELDNQIVTAT QSNICDEDSA TETCYTYDRN KCYTAVVPLV YGGETKMVET ALTPDACYPD.

Product Science Overview

Introduction

Immunoglobulin J (IgJ), also known as the joining chain, is a crucial protein in the immune system. It is synthesized in B lymphocytes and plays a significant role in the formation of polymeric immunoglobulins, particularly IgM and IgA. The recombinant form of this protein, produced through genetic engineering techniques, has become an essential tool in research and therapeutic applications.

Structure and Function

IgJ is a 137-amino acid protein that serves two primary functions:

  1. Linking Immunoglobulin Monomers: It links IgM monomers to form pentamers and IgA monomers to form dimers. This polymerization is essential for the immunoglobulins’ stability and functionality.
  2. Binding to Secretory Component: IgJ binds these immunoglobulins to the secretory component, facilitating their transport across epithelial cells and into secretions such as saliva, tears, and mucosal surfaces .
Genetic and Molecular Characteristics

The gene encoding IgJ is located on chromosome 4q21 in humans. This region is known for its genetic variability, including polymorphisms that can be useful in genetic linkage studies . The IgJ gene is adjacent to the CRLZ1 gene, and their expression is regulated in a stage-specific manner. IgJ is expressed in plasma cells, while CRLZ1 is expressed in pre-B cells .

Recombinant Production

Recombinant IgJ is produced using various expression systems, including bacterial, yeast, and mammalian cells. The recombinant form retains the functional properties of the native protein, making it valuable for research and therapeutic purposes. The production process involves cloning the IgJ gene into an expression vector, transforming the host cells, and purifying the expressed protein.

Applications
  1. Research: Recombinant IgJ is used in studies to understand the structure and function of polymeric immunoglobulins. It helps in elucidating the mechanisms of immune response and the role of IgJ in immunoglobulin transport and stability.
  2. Therapeutics: Recombinant IgJ has potential therapeutic applications, particularly in enhancing the efficacy of immunoglobulin-based treatments. It can be used to produce more stable and functional immunoglobulin formulations for treating various immune disorders.

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