IFNW1 Human

IFN-Omega 1 Human Recombinant
Cat. No.
BT28408
Source
Escherichia Coli.
Synonyms

IFN omega-1, IFN alpha-II-1, IFNW1.

Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

IFN-Omega 1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 19.9kDa.
The IFN-Omega 1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction

IFN-Omega 1, a type I interferon, is produced by virus-infected leukocytes. It is similar to other Type I IFNs, including IFN-alpha and IFN-beta, in its antiviral and antiproliferative functions, as well as its signaling pathway, which involves the IFNAR-1/IFNAR-2 receptor complex. IFN-Omega 1 shares about 75% of its amino acid sequence with IFN-alpha. The presence of two conserved disulfide bonds in its structure is crucial for its biological activity.

Description

Recombinant Human IFN-Omega 1, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 172 amino acids, resulting in a molecular weight of 19.9kDa. The purification process involves proprietary chromatographic techniques to ensure the protein's purity.

Physical Appearance
It appears as a sterile, filtered white powder that has been lyophilized (freeze-dried).
Formulation
The protein is lyophilized from a concentrated solution (0.2µm filtered) in PBS with a pH of 7.4.
Solubility
To reconstitute the lyophilized IFNW1, it is recommended to dissolve it in sterile 18M-cm H2O to a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized IFNW1 remains stable at room temperature for up to 3 weeks; however, it should be stored in a dry environment below -18°C for long-term storage. Once reconstituted, IFNW1 can be stored at 4°C for 2-7 days. For extended storage, freezing at -18°C is recommended. It is advisable to add a carrier protein, such as HSA or BSA, at a concentration of 0.1% to enhance stability during storage. Repeated freezing and thawing should be avoided to maintain protein integrity.
Purity
The purity of IFNW1 is greater than 95%, confirmed by two methods: Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).
Biological Activity
The biological activity of IFNW1 was assessed using a cytotoxicity assay on Human TF-1 cells. The ED50, which represents the concentration of the protein required to achieve 50% cytotoxicity, was determined to be less than 0.01 ng/ml. This corresponds to a specific activity greater than 100,000,000 units/mg.
Synonyms

IFN omega-1, IFN alpha-II-1, IFNW1.

Source
Escherichia Coli.
Amino Acid Sequence
MCDLPQNHGL LSRNTLVLLH QMRRISPFLC LKDRRDFRFP QEMVKGSQLQ KAHVMSVLHE MLQQIFSLFH TERSSAAWNM TLLDQLHTGL HQQLQHLETC LLQVVGEGES AGAISSPALT LRRYFQGIRV YLKEKKYSDC AWEVVRMEIM KSLFLSTNMQ ERLRSKDRDL GSS.

Product Science Overview

Discovery and Structure

IFN-Omega 1 was discovered relatively recently compared to other interferons. It is naturally occurring in the human body and is produced through genetic engineering for research and therapeutic purposes . The protein consists of 172 or 174 amino acids and has an apparent molecular mass of about 25 kDa . It contains a single carbohydrate group, which is mainly composed of biantennary complex oligosaccharides with variable amounts of N-acetyl neuraminic acid .

Gene and Expression

The gene encoding IFN-Omega 1 is located on chromosome 9, along with other type I interferon genes . However, only one of these genes is functional, giving rise to the IFN-Omega protein, while the others are non-functional pseudogenes . The IFN-Omega gene is not expressed in unstimulated cells; its expression is induced by viral infections .

Biological Activity

IFN-Omega 1 exhibits about 75% sequence homology with IFN-Alpha and contains two conserved disulfide bonds that are crucial for its biological activities . It signals through the IFNAR-1/IFNAR-2 receptor complex, similar to other type I interferons, and exerts antiviral and antiproliferative activities . In quantitative terms, IFN-Omega is a significant component of human leukocyte interferon, contributing to its total antiviral activity .

Therapeutic Potential

The therapeutic potential of IFN-Omega 1 is still being explored. Its ability to modulate immune responses and inhibit viral replication makes it a promising candidate for treating various viral infections and possibly certain cancers . However, more research is needed to fully understand its physiological role and therapeutic applications .

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