IFNA7 Human, Sf9

Interferon-alpha 7 (IFNA7) is a member of the alpha/beta interferon family, which plays a crucial role in the immune response against viral infections. Interferons are cytokines produced by host cells in response to pathogens such as viruses, bacteria, and parasites. They are named for their ability to “interfere” with viral replication within host cells.

The recombinant form of Interferon-alpha 7 is produced in Sf9 Baculovirus cells. Sf9 cells are derived from the fall armyworm (Spodoptera frugiperda) and are commonly used in biotechnology for the production of recombinant proteins. The recombinant IFNA7 produced in these cells is a single, glycosylated polypeptide chain containing 175 amino acids, with a molecular mass of approximately 20.7 kDa .

The recombinant IFNA7 protein includes a 6 amino acid His-Tag at the C-terminus, which facilitates its purification through chromatographic techniques. The protein is typically formulated in a phosphate-buffered saline solution with 10% glycerol to ensure stability. It is recommended to store the protein at 4°C for short-term use and at -20°C for long-term storage to avoid multiple freeze-thaw cycles .

Interferon-alpha 7 exhibits antiviral activities by promoting the production of two key enzymes: a protein kinase and an oligoadenylate synthetase. These enzymes play a role in inhibiting viral replication and enhancing the immune response. IFNA7 is produced by macrophages and is involved in various immune processes, including B cell activation and lymphocyte activation .

Recombinant IFNA7 is used extensively in laboratory research to study its antiviral properties and its role in the immune response. It is also used in various assays to investigate the signaling pathways mediated by interferons and their potential therapeutic applications in treating viral infections and certain types of cancer .